PCR Reagents
Absolute Master Mix
Absolute Master Mix (AMM) is a ready-to-use PCR reaction mixture. It only requires addition of primers, DNA template and water to carry out polymerase chain reaction. AMM contains Taq DNA polymerase, PCR buffer, dNTPs and loading dyes. Absolute Master Mix is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions.
Absolute Master Mix II
Absolute Master Mix II (AMM II) is a ready-to-use PCR reaction mixture. It only requires addition of primers, DNA template and water to carry out polymerase chain reaction. AMM II contains Taq DNA polymerase, PCR buffer, dNTPs, loading dyes, enhancer and fluorescence dye. It is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions.
Hot Start Master Mix
HS Master Mix is a ready-to-use PCR reaction mixture. It only requires addition of primers, DNA template and water to carry out polymerase chain reaction. The 2X HS Master Mix contains DNA Polymerase blend (0.2U/μl reaction), reaction buffer (1X), dNTPs (0.4 mM of each dNTP at 1X), MgCl2 (4 mM at 1X), stabilizers and DNA loading dyes. The enzyme contains a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the initial denaturation step in PCR cycling at 94°C, thereby providing an automatic “hot start” for Taq DNA polymerase in PCR.
dNTPs Mix
dNTPs Mix (PCR Grade) offered by MOLEQULE-ON, consists of all four nucleotides (dATP, dCTP, dGTP, dTTP), each at a concentration of 25mM, in a solution of 0.6mM Tris-HCl (pH 7.5). The 25mM dNTP Mix is suitable for use in polymerase chain reaction (PCR), sequencing, filling, nick translation, cDNA synthesis, and TdT-tailing reactions. dNTPs Mix can be diluted to the desired concentration. Highly pure dNTPs are important for successful PCR, as the presence of contaminating impurities in PCR can result in a decrease in amplification sensitivity and product yield.
Taq DNA Polymerase
Taq DNA Polymerase is a thermostable DNA polymerase that catalyzes the polymerization of nucleotides into duplex DNA in the 5’ →3’ direction. MOLEQULE-ON Taq DNA polymerase is isolated from E. coli strain containing the Taq DNA polymerase gene from Thermus aquaticus. The buffer system is optimized for high specificity and guarantees minimal by-product formation. 10X reaction buffer included MgCl2 thus making the master mix preparation easy and error free.
