Taq DNA Polymerase
Taq DNA Polymerase is a thermostable DNA polymerase that catalyzes the polymerization of nucleotides into duplex DNA in the 5’ →3’ direction. MOLEQULE-ON Taq DNA polymerase is isolated from E. coli strain containing the Taq DNA polymerase gene from Thermus aquaticus. The buffer system is optimized for high specificity and guarantees minimal by-product formation. 10X reaction buffer included MgCl2 thus making the master mix preparation easy and error free.
Proteinase K is a broad-spectrum serine protease useful for general digestion of proteins. MOLEQULE-ON Proteinase K is highly active and stable in SDS, EDTA, urea and at high temperatures. Its activity is greater than 40 units/mg at 37°C. Proteinase K can be used in the preparation of RNA and high molecular weight DNA. It can also be used in the preparation of tissue sections for in situ hybridization, inactivation of nucleases during nucleic acid extraction and lysis of cells to facilitate isolation of intact genomic DNA or RNA.
Ribonuclease A (RNase A) is an endoribonuclease that attacks at the 3’OH phosphate of a pyrimidine nucleotide. MOLEQULE-ON RNase A is used to remove RNA from genomic DNA and plasmid preparations. It is also used in RNA sequence analysis and protection assays.
Restriction enzymes recognize short DNA sequences and cleaves double-stranded DNA at or near a specific recognition site. MOLEQULE-ON offers a variety of Restriction Enzymes with different buffer system that allow user to choose the correct buffer according to experimental need. .