MQ Basic cDNA Synthesis Kit
The enzyme Reverse transcriptase has the ability to reverse the process of translation from DNA to RNA and this step has been a crucial application of modern molecular biology. Reversing the messenger RNA (mRNA) delivers thecomplementary DNA (cDNA), which can be detected or quantified using PCR and qPCR approaches. The Basic cDNA Synthesis Kit from MOLEQULE-ON offers all necessary components to convert RNA into cDNA in one kit including oligo dT and random hexamer. The MQ Scriptase Basic is an enhanced version of the original MuLV Reverse transcriptase.
Advanced cDNA Synthesis Kit
Absolute Master Mix II (AMM II) is a ready-to-use PCR reaction mixture. It only requires addition of primers, DNA template and water to carry out polymerase chain reaction. AMM II contains Taq DNA polymerase, PCR buffer, dNTPs, loading dyes, enhancer and fluorescence dye. It is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions.
Hi-Tech Green 2X qPCR Universal Mix
Through extensive research, an intercalating DNA dye has been developed which is suitable for real-time quantification of amplified DNA without inhibiting the polymerization reaction, often seen with other popular intercalating dyes. Hi-Tech Green has an optimized buffer mixture, able to efficiently amplify GC- and AT-rich using standard or fast cycling conditions. Unspecific signal detection and lower amplification efficiency originated from primer-dimers are inhibited using small molecule inhibition. This ready-to-use mix can replace any commercial Dye based qPCR mixture and is compatible with most of the well-known instruments. Hi-Tech Green qPCR mix has a dye that does not inhibit PCR and it comes with separate ROX vial for addition to the master mix. Hi-Tech Green qPCR Mix is ideally suited for gene expression analysis (absolute and relative), detection of low copy genes and quantification of viral loads or NGS libraries.
MQ Probe 2X qPCR Mix
Through extensive research, a buffer mixture has been created which is optimal for the detection of multiple fluorophores within a mixture. This research also led to the development of buffer chemistries optimized for earlier CT-values in a qPCR assay. Combining the earlier CT-values with the multiplex, the MQ Probe 2X qPCR Universal Mix is perfect for qPCR assays using Probes. This ready-to-use mix can replace any commercial Probe based qPCR mixture and is compatible with most of the well-known instruments. MQ Probe qPCR mix has to be optimized for multiplex qPCR and it comes with separate ROX vial for addition to the master mix. MQ Probe qPCR Mix is ideally suited for multiplex qPCR, gene expression analysis (absolute and relative), and detection of low copy genes and quantification of viral loads or NGS libraries.
MQ One Step Probe qPCR Mix
MQ One step Probe qPCR Mix offers two separate reactions in one step i.e., cDNA Synthesis and Real-time PCR. It combines the reverse transcription with a qPCR detection mix, which results in the optimal convenience protocol therefore lowering the risk of contamination by avoiding additional pipetting. The reverse transcriptase implemented in this kit is a modified MuLV, engineered to fasten the process of turning the RNA to DNA. This enables a 10 min reverse transcription rather than a 1-hour step using the wild type enzyme. Extensive research allowed us to create a buffer mixture optimal for the detection of multiple fluorophores within a mixture. This research also led to the development of buffer chemistries optimizied for earlier CT -values in a qPCR assay. Combining the earlier CT -values with the multiplex, the MQ One Step Probe qPCR Mix is perfect for qPCR assays using Probes.