Electrophoresis Reagents
Agarose E
MOLEQULE-ON Agarose E is ideal for routine rapid separations of DNA and RNA fragments as well as PCR products, the preparation of plasmids, and for screening, cloning and blotting techniques. Agarose E has high gel strength even at low concentrations, so use rates are 0.75 – 2%. It is effective in blotting and in separations of nucleic acid fractions from 250 base pair to 23 kilo bases.
VisualaNA (Nucleic Acid Staining Dye)
VisualaNA A (Nucleic Acid Staining Dye)
MOLEQULE-ON offers VisualaNA A dye, which is used to visualize DNA or RNA in electrophoresis. It is a safe alternative to the traditional ethidium bromide stain. It is a non-carcinogenic and less mutagenic dye for detecting dsDNA, ssDNA and RNA in agarose gels with a very high sensitivity. VisualaNA dyes are compatible with UV light or with Blue/Green LED technology. This dye is added in small volume in agarose gel for detection of nucleic acids. Several test have been performed on VisualaNA to determined its safety towards user; like Ames test, Acute oral toxicity test, Mouse bone marrow micronucleaus test, Chromosome aberration test and latex and nitrile gloves penetration test and according to these tests VisualaNA A is a safe dye.
VisualaNA S (Nucleic Acid Staining Dye)
MOLEQULE-ON offers VisualaNA S dye, which is used to visualize DNA or RNA in electrophoresis. It is a safe alternative to the ethidium bromide stain. It is a non-carcinogenic and less mutagenic dye for detecting dsDNA, ssDNA and RNA in agarose gels with a very high sensitivity. VisualaNA dyes are compatible with UV light or with Blue/Green LED technology. VisualaNA S is added in sample in order to increase the intensity of dye and detected on system. Several test have been performed on VisualaNA to determined its safety towards user; like Ames test, Acute oral toxicity test, Mouse bone marrow micronucleaus test, Chromosome aberration test and latex and nitrile gloves penetration test and according to these tests VisualaNA S is a safe dye.
VisualaNA X (Nucleic Acid Staining Dye)
VisualaNA X is a new member to VisualaNA family. It is a safe nucleic acid stain for the detection of double stranded DNA, single-stranded DNA and RNA in agarose gels. This dye replaces Ethidium Bromide (toxic and potential mutagen) for visualization of DNA and RNA in agarose gel. VisualaNA X DNA stain emits green fluorescence when bound to DNA or RNA. It has two fluorescent excitation maxima of ~250 and ~482 nm, and an emission maximum of ~509 nm. VisualaNA X was developed to work best with Blue and Blue/Green LED Illuminators and Gel Doc Systems.
DNA Loading Dye
DNA Loading Dye is a pre-mixed loading buffer with two tracking dyes and supplied as 6X concentration. It is used for agarose and non-denaturating polyacrylamide gel electrophoresis. Bromophenol blue is the standard tracking dye for electrophoresis. It migrates at approximately 300 base pairs on 1% TBE agarose gel. Whereas, xylene cyanol typically migrates at about the same rate as a 4000 base pairs DNA fragment. EDTA is also included to chelate magnesium (Mg2+) in enzymatic reactions, thereby stopping the reaction.
DNA Markers
50bp DNA Marker
MOLEQULE-ON 50bp DNA Marker is a unique combination of PCR product and a number of proprietary plasmids digested with appropriate restriction enzymes. It separates into 17 fragments, suitable for use as molecular weight standards for horizontal and vertical gel electrophoresis. The DNA includes fragments ranging from 50-1,500 base pairs. The 200 and 500 base pair bands have increased intensity to serve as reference points.
100bp DNA Marker
MOLEQULE-ON 100bp DNA Marker is a unique combination of PCR product and a number of proprietary plasmids digested with appropriate restriction enzymes. It separates into 11 fragments, suitable for use as molecular weight standards for horizontal and vertical gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points.
1kb DNA Marker
MOLEQULE-ON 1Kb DNA Marker is a unique combination of PCR product and a number of proprietary plasmids digested with appropriate restriction enzymes. It separates into 13 fragments, suitable for use as molecular weight standards for horizontal and vertical gel electrophoresis. The DNA includes fragments ranging from 250 – 10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points.
1kb Plus DNA Marker
MOLEQULE-ON 1KB Plus DNA Marker is a unique combination of PCR product and a number of proprietary plasmids digested with appropriate restriction enzymes. It separates into 19 fragments, suitable for use as molecular weight standards for horizontal and vertical gel electrophoresis. The DNA includes fragments ranging from 100 to 10,000 base pairs. The 500, 1.5K and 3K base pair bands have increased intensity to serve as reference points.
1kb XL DNA Marker
MOLEQULE-ON KB XL DNA Marker is a unique combination of PCR product and a number of proprietary plasmids digested with appropriate restriction enzymes. It separates into 14 fragments, suitable for use as molecular weight standards for horizontal and vertical gel electrophoresis. The DNA includes fragments ranging from 250-25K base pairs. The 1K and 3K base pair bands have increased intensity to serve as reference points.
Protein Markers
Blue Prestained Protein Marker
The MOLEQULE-ON Blue Protein Marker is a three-color standard with 12 pre-stained proteins, covering a wide range of molecular weights from 10 to 245 kDa. It is ready-to-use product, supplied in a loading buffer for direct loading on gels. Easy to identify with the reference bands at ~25 and ~90 kDa coupled with green and red chromophore respectively.