Easy Protein Gels
MOLEQULE-ON offers pre-cast polyacrylamide gels that are ready to run and designed to give optimal separation of small- to medium-sized proteins. Easy protein gels provide high quality and reliability and eliminate exposure to toxic unpolymerized acrylamide. Use Easy Protein Gels for preparing proteins for SDS-PAGE, native PAGE, peptide separations, nucleic acid separations, western blotting, sequencing, mass spectrometry, and any other techniques where protein integrity is required.
BCA Protein Assay Kit
The BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation using a unique reagent containing bicinchoninic acid. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range (20-2,000 μg/ml).