RNA KITS
MQ Basic RNA Isolation Kit
The MQ Basic RNA Isolation Kit purifies total RNA samples from mammalian tissues and cultured cells. The isolated RNA can be used for a variety of downstream applications e.g. RT-PCR, qPCR, cDNA synthesis, northern blot, next generation sequencing and much more. In order to prevent RNA degradation, the sample is treated right at the beginning with an RNases inhibitory lysis buffer. This step ensures purification of intact RNA. Addition of ethanol provides appropriate RNA binding conditions to the silica membrane of the MQ binding column. In following steps contaminations are efficiently washed away with the supplied buffers from the column. High-quality RNA is subsequently eluted in buffer. The purified RNA is ready for downstream applications or storage.
MQ Advanced RNA Isolation Kit
The MQ Advanced RNA Isolation Kit purifies total RNA samples from mammalian tissues and cultured cells. The isolated RNA can be used for a variety of downstream applications e.g. RT-PCR, qPCR, cDNA synthesis, northern blot, next generation sequencing and much more. In order to prevent RNA degradation the sample is treated right at the beginning with an RNases inhibitory lysis buffer. This step ensures purification of intact RNA. Addition of ethanol provides appropriate RNA binding conditions to the silica membrane of the MQ binding column. In following steps contaminations are efficiently washed away with the supplied buffers from the column. High-quality RNA is subsequently eluted in buffer. The purified RNA is ready for downstream applications or storage.
The MQ Advanced RNA Isolation Kit guarantees pure high-quality RNA due to an optimized DNase I treatment in combination with a specifically engineered MQ mini-elute column technology. Unlike in kits of other suppliers the DNase I treatment will take place in liquids and not on top of the column membrane. This increases the DNase I efficiency a lot. Following RNA is bound to the membrane of the MQ mini-elute column that possesses a high RNA binding capacity. A much higher RNA concentration can be reached due to the small column diameter, so that elution volume can be decreased to 10μl.
MQ Virus Nucleic Acid Isolation Kit
The MQ Virus Nucleic Acid Isolation Kit provides a fast, simple, and cost-effective method for the isolation of viral DNA/RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of virus-infected cell cultures. Its unique buffer system will efficiently lyse cells and degrade protein, allowing for the nucleic acid to be easily bound by the glass fiber matrix of the column. Contaminants such as salts, metabolites and soluble macromolecular cellular components are removed in the Wash Step. High quality nucleic acid is eluted in the RNase-free elution buffer. This kit is suitable for a variety of routine applications, including the Real-time PCR/RT-PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions. The entire procedure can be completed within 15-20 minutes.