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Western Blot

MOLEQULE-ON reagents and kits are essential tool in research and various field of science, which enable accurate experiments and tests. Our products are integrated with newest technologies to meet the demand of a wide range of laboratories. The list includes PCR and qPCR reagents, electrophoresis, enzymes, nucleic acid extraction and purification kits, colorimetric and ELISA kits and buffers.

Western Blot
MOLEQULE-ON is a provider of Biochemistry, Microbiology and Biotechnology products. It Includes equipment, kits,
PCR reagents, dehydrated culture media and plasticware. Our company is focused on customer satisfaction striving to
gain the reputation of a reliable supplier of user-friendly laboratory tools.

Easy Protein Gel

Description

MOLEQULE - ON offers pre - cast polyacrylamide gels that are ready to run and designed to give optimal separation of small to medium - sized proteins. Easy protein gels provide high quality and reliability and eliminate exposure to toxic unpolymerized acrylamide. Use Easy Protein Gels for preparing proteins for SDS - PAGE, native PAGE, peptide separations, nucleic acid separations, western blotting, sequencing, mass spectrometry, and any other techniques where protein integrity is required. A pack of running buffer is included in Easy Protein Gel package (one box of 10 gels).

Specification

1. No. of wells: 10 wells
2. Outer gel dimension: 8cm x 10 cm
3. Thickness of gel layer: 1.5 mm
4. Maximum loading volume of gel well: 25 μl
5. Gradient: Tris HEPES Gradient (4 - 20 %)

Compatibility

Easy Protein Gel is suitable for the most of mini SDS - PAGE electrophoresis tank, including
1. Bio - Rad Mini - PROTEAN (II/3 /Tetra System)
2. Hoefer Mighty Small (SE 250/ SE 260/ SE 280)
3. Life Technology Novex Mini - Cell (use with special baffle)

Ordering Information
Product Name
Easy Protein Gel
Catalog Number
EPG-M-001-10

MQ Western One Step Blocker

Description

MQ Western One Step Blocker is a blocking solution for Western blot analysis. This Blocker buffer not only provides blocking and primary and secondary antibody hybridization in one step but also enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. It, therefore, serves as both blocker and enhancer in Western analysis. With the three - in - one step procedure, MQ Western One Step Blocker is a time and labor economic solution for the time consuming and laborious Western procedure.

Features

  • 3 Steps in One: Block the membrane and dilute primary and secondary antibody in one step.
  • Enhance antibody signal: It shows a two-to five-fold increase in signal intensity for most protein targets, enabling much less protein to be detected with the same substrate and method.
  • Enhance time-saving: It saves at least 2 hours in the antibody detection process during the Western Blot, with only one hour needed.
  • Universal antibody diluent: Ready-to-use dilution buffer for most of primary and secondary antibody.
  • No blocking step needed: Just immerse the membrane in the MQ Western One Step Blocker solution with your antibodies.
  • Effective with any ECL: After the antibody detection process, the signal can be developed with both HRP (horseradish peroxidase) and AP (alkaline phosphatase) substrates.
  • Less hands-on steps: No 3 wash steps are required, meaning no need to transfer the membrane in & out of the container.
  • Compatible with PVDF & NC membrane: Regardless of the pore size, the MQ Blocker minimizes the background from non - specific protein binding by antibodies.
  • Improve protein detection: Improve the binding process of target proteins, so that specific antibodies can bind more effectively.
  • Protein free: Reduces overall background and minimizes non-specific signals often seen with ECL detection.
  • 1°Ab (ab 8227, abcam ): Rabbit anti beta actin; 1:5,000
    2°Ab (ab 205718, abcam ): Goat anti Rabbit HRP; 1:5,000

    Ordering Information
    Product Name
    MQ Western One Step Blocker
    Catalog Number
    WOB-M-500

    MQ Western Stripping Buffer

    Description

    Nitrocellulo se and PVDF membranes probed by Western blotting procedures and detected by chemiluminescent or other non - precipitating substrates can be stripped and re - probed using MQ Western Stripping Buffer. Western blotting is widely used to detect and compare proteins in complex mixtures, and chemiluminescence has largely replaced chromogenic substrates as the most convenient and sensitive method of detection. One advantage of chemiluminescence is the ability to strip and re - probe the protein mixture on the membrane. Traditional stripping methods use conditions that are effective for only low - affinity antibody - antigen interactions or are so harsh that they tend to adversely alter the antigen for subsequent immunoprobing. MQ Western Stripping Buffer is a robust but gentle formulation for stripping primary and secondary antibodies from blots to enable several reprobings on the same membrane.

    Ordering Information
    Product Name
    MQ Western Stripping Buffer
    Catalog Number
    BF-M-WSB-500

    Tris Buffered Saline Buffer, TBS, (20X)

    Description

    Tris Buffered Saline (TBS) is a buffering solution that stabilizes pH. The solution is commonly used for western blot and ELISA procedures. It is used as a convenient prepared buffer for use in various applications.

    Working Solution

    To make one liter of 1X working concentration, take 50ml of 20X TBS Buffer and mix with 950ml of sterile distilled water. 1X Buffer contains 0.14M Sodium Chloride, 3mM Potassium Chloride and 25mM Tris Base.

    Ordering Information
    Product Name
    Tris Buffered Saline Buffer, TBS, (20X)
    Catalog Number
    BF-M-TBS-20X
    Protein Staining Solution

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    Ponceau S Protein Staining Solution

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    Western chemiluminescence Substrate

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    PBS Buffer

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    Easy Protein Gels

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    MQ Western One Step Blocker

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    MQ Western Stripping Buffer

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    Tris Buffered Saline Buffer, TBS, (20X)

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    Promising Best Quality Services
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    Lab
    Designing

    Lab
    Furniture

    Matrix
    Cleanroom

    DNA
    Sequencing

    Oligo
    Synthesis

    Promising Best Quality Services
    Our customized services

    Lab
    Designing

    Lab
    Furniture

    Matrix
    Cleanroom

    DNA
    Sequencing

    Oligo
    Synthesis

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