Electrophoresis Reagents
MOLEQULE-ON reagents and kits are essential tool in research and various field of science, which enable accurate experiments and tests. Our products are integrated with newest technologies to meet the demand of a wide range of laboratories. The list includes PCR and qPCR reagents, electrophoresis, enzymes, nucleic acid extraction and purification kits, colorimetric and ELISA kits and buffers.
50bp DNA Ladder
MOLEQULE-ON 50 bp DNA Marker is a unique combination of PCR products and several proprietary plasmids digested with appropriate restriction enzymes to yield 17 fragments. These fragments are suitable for use as molecular weight standards for both horizontal and vertical gel electrophoresis.
The DNA fragments range from 50 to 1,500 base pairs. The 200 and 500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.56 μg per load) for estimating the mass of DNA in samples of similar intensity and size.
MOLEQULE-ON 50 bp DNA Marker also contains Orange G as a tracking dye.
Product Name
50bp DNA Ladder
Catalog Number
DM-M-001-500
100bp DNA Ladder
MOLEQULE-ON 100 bp DNA Marker is a unique combination of PCR products and several proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments. These fragments are suitable for use as molecular weight standards for both horizontal and vertical gel electrophoresis.
The DNA includes fragments ranging from 100 to 1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg per load) for estimating the mass of DNA in samples of similar intensity and size.
MOLEQULE-ON 100 bp DNA Marker contains Orange G and xylene cyanol FF as tracking dyes.
Product Name
100bp DNA Ladder
Catalog Number
DM-M-002-500
100bp Plus DNA Ladder
MOLEQULE-ON 100 bp Plus DNA Marker is a unique combination of PCR products and several proprietary plasmids digested with appropriate restriction enzymes to yield 19 fragments. These fragments are suitable for use as molecular weight standards for both horizontal and vertical gel electrophoresis.
The DNA includes fragments ranging from 100 to 10,000 base pairs. The 500, 1.5K, and 3K base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.86 μg per load) for estimating the mass of DNA in samples of similar intensity and size.
MOLEQULE-ON 100 bp Plus DNA Marker contains bromophenol blue and xylene cyanol FF as tracking dyes.
Product Name
100bp Plus DNA Ladder
Catalog Number
DM-M-002-500
1kb DNA Ladder
MOLEQULE-ON 1 Kb DNA Marker is a unique combination of PCR products and several proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments. These fragments are suitable for use as molecular weight standards for both horizontal and vertical gel electrophoresis.
The DNA includes fragments ranging from 250 to 10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg per load) for estimating the mass of DNA in samples of similar intensity and size.
MOLEQULE-ON 1 Kb DNA Marker contains bromophenol blue and xylene cyanol FF as tracking dyes.
Product Name
1kb DNA Ladder
Catalog Number
DM-M-003-500
1kb Plus DNA Ladde
MOLEQULE-ON 1 KB Plus DNA Marker is a unique combination of PCR products and several proprietary plasmids digested with appropriate restriction enzymes to yield 19 fragments. These fragments are suitable for use as molecular weight standards for both horizontal and vertical gel electrophoresis.
The DNA includes fragments ranging from 100 to 10,000 base pairs. The 500, 1.5K, and 3K base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.86 μg per load) for estimating the mass of DNA in samples of similar intensity and size.
MOLEQULE-ON 1 KB Plus DNA Marker contains bromophenol blue and xylene cyanol FF as tracking dyes.
Product Name
1kb Plus DNA Ladder
Catalog Number
DM-M-004-500
MQ Blue Protein Marker
Description
The MOLEQULE-ON Blue Protein Marker is a three-colour standard with 12 pre-stained proteins, covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands, when separated on SDS-PAGE (Tris-glycine buffer). The Blue Marker is dissolved in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol).
Features
1. Broad range: 10-245 kDa
2. Ready-to-use: Supplied in a loading buffer for direct loading on gels
3. Easy to identify: Features reference bands at ~25 and ~90 kDa, coupled with green and red chromophores, respectively
4. Sharp band
Applications
1. Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
2. Monitoring of protein transfer onto membranes during Western blotting.
3. Sizing of proteins on SDS-polyacrylamide gels and Western blots.
Product Name
MQ Blue Protein Marker
Catalog Number
PM-M-002-500
Agarose
Principles and Uses
Agarose E is an agarose ideal for routine rapid separation of DNA and RNA fragments, as well as PCR products, plasmid preparation, and for screening, cloning, and blotting techniques. Some important features are:
1. Easy dissolution and rapid gelling.
2. Excellent transparency and low background staining for clear band visibility.
3. Sharp and well-defined bands.
4. Very low DNA binding.
Agarose E has high gel strength even at low concentrations, so use rates are 0.75-2%. It is effective in blotting and in separating nucleic acid fractions from 250 bp to 23 Kb.
Physical-Chemical Characteristics
Product Name
Agarose
Catalog Number
CH-M-001-100
6X DNA Loading Dye
The MOLEQULE-ON DNA Loading Dye is a pre-mixed loading buffer with two tracking dyes for agarose and non-denaturing polyacrylamide gel electrophoresis. Bromophenol blue is the standard tracking dye for electrophoresis, migrating at approximately 300 base pairs on a 1% TBE agarose gel. Xylene cyanol typically migrates at about the same rate as a 4000 base pairs DNA fragment. EDTA is also included to chelate magnesium (Mg²⁺) in enzymatic reactions, thereby stopping the reaction. The DNA loading buffer with tracking dyes is supplied in a 6X concentrated form.
Product Name
6X DNA Loading Dye
Catalog Number
DY-M-001-1000
VisualaNA (A) Nucleic Acid Stain
Description
VisualaNA A is a safe nucleic acid stain for the detection of double-stranded DNA, single-stranded DNA, and RNA in agarose gels.
This dye replaces Ethidium Bromide (a toxic and potential mutagen) for visualization of DNA and RNA in agarose gel. VisualaNA A DNA stain emits green fluorescence when bound to DNA or RNA. It has two secondary fluorescence excitation peaks (~270 nm; ~290 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is centered at ~530 nm. Thus, VisualaNA A DNA stain is compatible with a wide variety of gel reading instruments. VisualaNA A can be used for precast agarose gels, and for better sensitivity, post-staining is recommended. VisualaNA A is non-carcinogenic and, according to the Ames test, causes significantly fewer mutations than Ethidium Bromide.
Features
1. Non carcinogenic
2. Non hazardous to the environment
3. VisualaNA A is as sensitive as ethidium bromide
VisualaNA Compatibility
VisualaNA A has been tested for use with the following applications: In gel staining, transformation, post-staining, ligation, gel extraction and transfection. Prior to setting, VisualaNA A/Agarose solution can be retained at 65°C for same day use.
VisualaNA A is non-carcinogenic but may cause skin and eye irritations. Always wear gloves when working with the product.
Product Name
VisualaNA (A) Nucleic Acid Stain
Catalog Number
DY-M-001-500
SDS-PAGE Sample Loading Buffer (4X)
Composition
Final concentrations in 1X buffer are 50 mM Tris-HCl pH 6.8, 2% SDS, 10% glycerol, 1% β-mercaptoethanol, 12.5 mM EDTA, and 0.02% bromophenol blue.
Product Name
SDS-PAGE Sample Loading Buffer (4X)
Catalog Number
CH-M-254
2X SDS-PAGE Sample Loading Buffer
Description
Description
MOLECULE-ON SDS-PAGE Sample Loading Buffer in 2X concentration is used for preparation and loading of protein samples onto a gel for SDS-PAGE analysis (Western blot/protein blot). SDS contained in the sample buffer makes proteins negatively charged proportionally to their length. β-mercaptoethanol breaks disulfide bonds, whereas ficoll makes the sample buffer more dense than the surrounding running buffer of the protein gel, enabling easy loading into the gel wells.
Composition
Final concentrations in 1X buffer are 50 mM Tris-HCl pH 6.8, 2% SDS, 10% ficoll, 1% β-mercaptoethanol, 12.5 mM EDTA, and 0.02% bromophenol blue.
Product Name
2X SDS-PAGE Sample Loading Buffer
Catalog Number
CH-M-436
4X Native PAGE Sample Loading Buffer
Product Name
4X Native PAGE Sample Loading Buffer
Catalog Number
CH-M-437
Tris Borate EDTA, TBE Buffer, 10X
TBE buffer (Tris-Borate-EDTA), 10X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA. 10X solution of TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
A single-strength (1X) solution contains 0.089M Tris base, 0.089M Borate, and 0.002M EDTA.
Product Name
Tris Borate EDTA, TBE Buffer, 10X
Catalog Number
BF-M-TBE-10X
Tris Borate EDTA Buffer, TBE, 5X
TBE buffer (Tris-Borate-EDTA), 5X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA. 5X solution of TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Product Name
Tris Borate EDTA Buffer, TBE, 5X
Catalog Number
BF-M-TBE-5X
Tris Borate EDTA Buffer, TBE, 1X
TBE buffer (Tris-Borate-EDTA), 1X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
A single-strength (1X) solution contains 0.089M Tris base, 0.089M Borate, and 0.002M EDTA.
Product Name
Tris Borate EDTA Buffer, TBE, 1X
Catalog Number
BF-M-TBE-1X
Tris Acetate EDTA Buffer, TAE, 10X
TAE (Tris-Acetate-EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double-stranded DNA runs faster with TAE buffer. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA and can also be used as both a running buffer and a gel preparation buffer. It is recommended for the resolution of RNA and DNA fragments larger than 1500 base pairs, for genomic DNA, and for large supercoiled DNA.
MOLEQULE-ON TAE Buffer is free from DNase, RNase, and Protease activity.
Preparation of 1X Working Solution
To prepare one liter of 1X working solution of TAE Buffer, mix 100 ml of 10X TAE Solution with 900 ml ultrapure water.
Product Name
Tris Acetate EDTA Buffer, TAE, 10X
Catalog Number
BF-M-TAE-10X
Tris Acetate EDTA Buffer, TAE, 25X
TAE (Tris-Acetate-EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double-stranded DNA runs faster with TAE buffer. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and also as both a running buffer and a gel preparation buffer. It is recommended for the resolution of RNA and DNA fragments larger than 1500 base pairs, for genomic DNA, and for large supercoiled DNA.
MOLEQULE-ON TAE Buffer is free from DNase, RNase, and Protease activity.
Product Name
Tris Acetate EDTA Buffer, TAE, 25X
Catalog Number
BF-M-TAE-25X
10X Tris-Glycine-SDS (TGS) Buffer
Tris-Glycine-SDS (TGS) running buffer is the most commonly used buffer for SDS-PAGE of proteins. TGS is usually used for both anode and cathode buffer. 10X Tris-Glycine-SDS Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-Glycine-SDS (pH 8.3) running buffer used for SDS-PAGE (protein polyacrylamide gel electrophoresis). Recommended running conditions are 150 volts for mini vertical gel electrophoresis units.
Product Name
10X Tris-Glycine-SDS (TGS) Buffer
Catalog Number
BF-M-TGS-10X
10X Tris-Glycine (TG) Buffer
Tris-Glycine-SDS (TGS) running buffer is the most commonly used buffer for native protein gel electrophoresis or transfer buffer for western blots. TGS buffer is usually used for both anode and cathode buffer. 10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.3) running buffer. It can be used for semi-dry blotting for SDS-PAGE gels by the addition of 20% methanol.
Recommended running conditions are 150 volts for mini vertical gel electrophoresis units.
Product Name
10X Tris-Glycine (TG) Buffer
Catalog Number
BF-M-TG-10X
Easy Protein Gels
1. No. of wells: 10 wells
2. Outer gel dimension: 8cm x 10 cm
3. Thickness of gel layer: 1.5 mm
4. Maximum loading volume of gel well: 25 μl
5. Gradient: Tris HEPES Gradient (4 – 20 %)
Easy Protein Gel is suitable for the most of mini SDS – PAGE electrophoresis tank, including
1. Bio – Rad Mini – PROTEAN (II/3 /Tetra System)
2. Hoefer Mighty Small (SE 250/ SE 260/ SE 280)
3. Life Technology Novex Mini – Cell (use with special baffle)
Product Name
Easy Protein Gels
Catalog Number
EPG-M-001-10
MQ Nano-Tech Protein Staining Solution (5X)
1. Clear background
2. Fast staining
3. No need for destaining
4. No washing step needed
5. No overstaining issue
6. No hazardous materials inside
7. No need to microwave or heat
Prestained protein ladder, unstained protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS -PAGE(4-20%), gel was removed from the cassette then proceed to submerge the gel in proper amount of MQ Nano – Tech Protein Staining Solution, enough to cover the gel. The staining box was lightly agitated for 5 minutes to over night at room temperature.
Analysis of 85 kDa band of lane 5 (red arrow) was done, The band intensity between different storage time at 37°C was compared. The variation is under 10%.
MQ Nano – Tech Protein Staining Solution demonstrates the high sensitivity detection could be down to 10 ng (Lane H). Prestained protein ladder, unstained protein ladder, and serial diluted BSA were prepared and applied in electrophoresis.
After running 10% homemade gel (0.75 mm thickness), please remove the gel from the cassette then proceed to submerge the gel in a proper amount of MQ Nano – Tech Protein Staining Solution, enough to cover the gel. Lightly agitate the staining container at room temperature when staining for 30 hrs.
MQ Nano – Tech Protein Staining Solution presents the equivalent or even superior performance on fast signals and clear background compared with other brands. Lightly agitate the staining box for 5 to 30 minutes at room temperature.
Product Name
MQ Nano-Tech Protein Staining Solution (5X)
Catalog Number
PSS-M-5X-500
MQ Nano-Tech Protein Staining Solution
MQ Nano – Tech Protein Staining Solution, improved by nano – technology, is a ready – to – use protein staining solution for SDS – PAGE gels. Its next generation formula offers a faster protein detection, higher sensitivity and there is no need for destaining. Also, the washing step can be omitted. In the absence of hazardous substances such as methanol and acetic acid, it is considered to be safe and environmentally friendly. MQ Nano – Tech Protein Staining Solution is also compatible with mass spectrophotometry.
1. Car background
2. Fast staining
3. No need for destaining
4. No washing step needed
5. No overstaining issue
6. No hazardous materials inside
7. No need to microwave or heat
MQ Nano – Tech Protein Staining Solution demonstrates the high sensitivity detection could be down to 10 ng (Lane H). Prestained protein ladder, unstained protein ladder, and serial diluted BSA were prepared and applied in electrophoresis.
After running 10% homemade gel (0.75 mm thickness), please remove the gel from the cassette then proceed to submerge the gel in a proper amount of MQ Nano – Tech Protein Staining Solution, enough to cover the gel. Lightly agitate the staining container at room temperature when staining for 30 hrs.
Product Name
Tris Borate EDTA, TBE Buffer, 10X
Catalog Number
BF-M-TBE-10X
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