MOLEQULE-ON reagents and kits are essential tool in research and various field of science, which enable accurate experiments and tests. Our products are integrated with newest technologies to meet the demand of a wide range of laboratories. The list includes PCR and qPCR reagents, electrophoresis, enzymes, nucleic acid extraction and purification kits, colorimetric and ELISA kits and buffers.
PCR reagents, dehydrated culture media and plasticware. Our company is focused on customer satisfaction striving to
gain the reputation of a reliable supplier of user-friendly laboratory tools.
- Protein Staining Solution
-
Ponceau S Protein
Staining Solution -
Western chemiluminescence
Substrate - PBS Buffer
- Easy Protein Gels
-
MQ Western One Step
Blocker -
MQ Western Stripping
Buffer -
Tris Buffered Saline
Buffer, TBS, (20X)
MQ Nano - Tech Protein Staining Solution
MQ Nano - Tech Protein Staining Solution, improved by nano - technology, is a ready - to - use protein staining solution for SDS - PAGE gels. Its next generation formula offers a faster protein detection, higher sensitivity and there is no need for destaining. Also, the washing step can be omitted. In the absence of hazardous substances such as methanol and acetic acid, it is considered to be safe and environmentally friendly. MQ Nano - Tech Protein Staining Solution is also compatible with mass spectrophotometry.
1. Car
background
2. Fast
staining
3. No
need
for
destaining
4. No
washing
step
needed
5. No
overstaining
issue
6. No
hazardous
materials
inside
7. No
need
to
microwave
or
heat
Prestained protein ladder, unstained protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS - PAGE (4 - 20%), gel was removed from the cassette then proceed to submerge the gel in proper amount of MQ Nano - Tech Protein Staining Solution, enough to cover the gel. The staining box was lightly agitated for 5 minutes to over night at room temperature.
Analysis of 85 kDa band of lane 5 (red arrow) was done, The band intensity between different storage time at 37°C was compared. The variation is under 10%.
MQ
Nano
-
Tech
Protein
Staining
Solution
demonstrates
the
high
sensitivity
detection
could
be
down
to
10
ng
(Lane
H).
Prestained
protein
ladder,
unstained
protein
ladder,
and
serial
diluted
BSA
were
prepared
and
applied
in
electrophoresis.
After
running
10%
homemade
gel
(0.75 mm
thickness),
please
remove
the
gel
from
the
cassette
then
proceed
to
submerge
the
gel
in
a
proper
amount
of
MQ
Nano
-
Tech
Protein
Staining
Solution,
enough
to
cover
the
gel.
Lightly
agitate
the
staining
container
at
room
temperature
when
staining
for
30
hrs.
MQ Nano - Tech Protein Staining Solution presents the equivalent or even superior performance on fast signals and clear background compared with other brands. Lightly agitate the staining box for 5 to 30 minutes at room temperature.
MQ Nano - Tech Protein Staining Solution (5X)
MQ Nano - Tech Protein Staining Solution, improved by nano - technology, is available as 5X concentrate used for SDS-PAGE gels. Its next generation formula offers a faster protein detection, higher sensitivity and there is no need for destaining. Also, the washing step can be omitted. In the absence of hazardous substances such as methanol and acetic acid, it is considered to be safe and environmentally friendly. MQ Nano-Tech Protein Staining Solution is also compatible with mass spectrophotometry.
1. Clear
background
2. Fast
staining
3. No
need
for
destaining
4. No
washing
step
needed
5. No
overstaining
issue
6. No
hazardous
materials
inside
7. No
need
to
microwave
or
heat
Prestained protein ladder, unstained protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS -PAGE(4-20%), gel was removed from the cassette then proceed to submerge the gel in proper amount of MQ Nano - Tech Protein Staining Solution, enough to cover the gel. The staining box was lightly agitated for 5 minutes to over night at room temperature.
Analysis of 85 kDa band of lane 5 (red arrow) was done, The band intensity between different storage time at 37°C was compared. The variation is under 10%.
MQ
Nano
-
Tech
Protein
Staining
Solution
demonstrates
the
high
sensitivity
detection
could
be
down
to
10
ng
(Lane
H).
Prestained
protein
ladder,
unstained
protein
ladder,
and
serial
diluted
BSA
were
prepared
and
applied
in
electrophoresis.
After
running
10%
homemade
gel
(0.75
mm
thickness),
please
remove
the
gel
from
the
cassette
then
proceed
to
submerge
the
gel
in
a
proper
amount
of
MQ
Nano
-
Tech
Protein
Staining
Solution,
enough
to
cover
the
gel.
Lightly
agitate
the
staining
container
at
room
temperature
when
staining
for
30
hrs.
MQ Nano - Tech Protein Staining Solution presents the equivalent or even superior performance on fast signals and clear background compared with other brands. Lightly agitate the staining box for 5 to 30 minutes at room temperature.
MQ Nano - Tech Protein Staining Solution
MQ Nano - Tech Protein Staining Solution, improved by nano - technology, is a ready - to - use protein staining solution for SDS - PAGE gels. Its next generation formula offers a faster protein detection, higher sensitivity and there is no need for destaining. Also, the washing step can be omitted. In the absence of hazardous substances such as methanol and acetic acid, it is considered to be safe and environmentally friendly. MQ Nano - Tech Protein Staining Solution is also compatible with mass spectrophotometry.
1. Car
background
2. Fast
staining
3. No
need
for
destaining
4. No
washing
step
needed
5. No
overstaining
issue
6. No
hazardous
materials
inside
7. No
need
to
microwave
or
heat
Prestained protein ladder, unstained protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS - PAGE (4 - 20%), gel was removed from the cassette then proceed to submerge the gel in proper amount of MQ Nano - Tech Protein Staining Solution, enough to cover the gel. The staining box was lightly agitated for 5 minutes to over night at room temperature.
Analysis of 85 kDa band of lane 5 (red arrow) was done, The band intensity between different storage time at 37°C was compared. The variation is under 10%.
MQ
Nano
-
Tech
Protein
Staining
Solution
demonstrates
the
high
sensitivity
detection
could
be
down
to
10
ng
(Lane
H).
Prestained
protein
ladder,
unstained
protein
ladder,
and
serial
diluted
BSA
were
prepared
and
applied
in
electrophoresis.
After
running
10%
homemade
gel
(0.75 mm
thickness),
please
remove
the
gel
from
the
cassette
then
proceed
to
submerge
the
gel
in
a
proper
amount
of
MQ
Nano
-
Tech
Protein
Staining
Solution,
enough
to
cover
the
gel.
Lightly
agitate
the
staining
container
at
room
temperature
when
staining
for
30
hrs.
MQ Nano - Tech Protein Staining Solution presents the equivalent or even superior performance on fast signals and clear background compared with other brands. Lightly agitate the staining box for 5 to 30 minutes at room temperature.
MQ Nano - Tech Protein Staining Solution (5X)
MQ Nano - Tech Protein Staining Solution, improved by nano - technology, is available as 5X concentrate used for SDS-PAGE gels. Its next generation formula offers a faster protein detection, higher sensitivity and there is no need for destaining. Also, the washing step can be omitted. In the absence of hazardous substances such as methanol and acetic acid, it is considered to be safe and environmentally friendly. MQ Nano-Tech Protein Staining Solution is also compatible with mass spectrophotometry.
1. Clear
background
2. Fast
staining
3. No
need
for
destaining
4. No
washing
step
needed
5. No
overstaining
issue
6. No
hazardous
materials
inside
7. No
need
to
microwave
or
heat
Prestained protein ladder, unstained protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS -PAGE(4-20%), gel was removed from the cassette then proceed to submerge the gel in proper amount of MQ Nano - Tech Protein Staining Solution, enough to cover the gel. The staining box was lightly agitated for 5 minutes to over night at room temperature.
Analysis of 85 kDa band of lane 5 (red arrow) was done, The band intensity between different storage time at 37°C was compared. The variation is under 10%.
MQ
Nano
-
Tech
Protein
Staining
Solution
demonstrates
the
high
sensitivity
detection
could
be
down
to
10
ng
(Lane
H).
Prestained
protein
ladder,
unstained
protein
ladder,
and
serial
diluted
BSA
were
prepared
and
applied
in
electrophoresis.
After
running
10%
homemade
gel
(0.75
mm
thickness),
please
remove
the
gel
from
the
cassette
then
proceed
to
submerge
the
gel
in
a
proper
amount
of
MQ
Nano
-
Tech
Protein
Staining
Solution,
enough
to
cover
the
gel.
Lightly
agitate
the
staining
container
at
room
temperature
when
staining
for
30
hrs.
MQ Nano - Tech Protein Staining Solution presents the equivalent or even superior performance on fast signals and clear background compared with other brands. Lightly agitate the staining box for 5 to 30 minutes at room temperature.
MQ Ponceau S Protein Staining Solution
MQ Ponceaus S Staining Solution is a ready - to - use membrane stain for evaluating the transfer efficiency of a western blot. This stain is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. Ponceau S staining is reversible and can be removed with a short incubation in 0.1% NaOH .
Ponceau
S
solution
can
be
used
to
evaluate
for
total
protein
amount
or
transfer
efficiency
on
nitrocellulose
and
PVDF
membrane.
The
PVDF
membrane
stained
Ponceau
S
for
5
mins
and
washed
with
ddH2O
for
3
mins.
It
provides
visible
pink
bands.
Lane
1,13:
Prestained
MQ
Protein
Ladder
Lane
2
-
7:
2X
Dilutions
of
Unstained
Protein
Ladder
Lane
8
-
12:
2000,
1000,
500,
100,
50
ng
of
BSA
MQ Ponceau S Protein Staining Solution (10X)
MQ Ponceaus S Staining Solution, 10X concentrate is a membrane stain for evaluating the transfer efficiency of a western blot. This stain is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. Ponceau S staining is reversible and can be removed with a short incubation in 0.1% NaOH.
Ponceau
S
solution
can
be
used
to
evaluate
for
total
protein
amount
or
transfer
efficiency
on
nitrocellulose
and
PVDF
membrane.
The
PVDF
membrane
stained
Ponceau
S
for
5
mins
and
washed
with
ddH2O
for
3
mins.
It
provides
visible
pink
bands.
Lane 1,
13:
Prestained
MQ
Protein
Ladder
Lane
2
-
7:
2X
Dilutions
of
Unstained
Protein
Ladder
Lane
8
-
12:
2000,
1000,
500,
100,
50
ng
of
BSA
MQ Ponceau S Protein Staining Solution
MQ Ponceaus S Staining Solution is a ready - to - use membrane stain for evaluating the transfer efficiency of a western blot. This stain is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. Ponceau S staining is reversible and can be removed with a short incubation in 0.1% NaOH .
Ponceau
S
solution
can
be
used
to
evaluate
for
total
protein
amount
or
transfer
efficiency
on
nitrocellulose
and
PVDF
membrane.
The
PVDF
membrane
stained
Ponceau
S
for
5
mins
and
washed
with
ddH2O
for
3
mins.
It
provides
visible
pink
bands.
Lane
1,13:
Prestained
MQ
Protein
Ladder
Lane
2
-
7:
2X
Dilutions
of
Unstained
Protein
Ladder
Lane
8
-
12:
2000,
1000,
500,
100,
50
ng
of
BSA
MQ Ponceau S Protein Staining Solution (10X)
MQ Ponceaus S Staining Solution, 10X concentrate is a membrane stain for evaluating the transfer efficiency of a western blot. This stain is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. Ponceau S staining is reversible and can be removed with a short incubation in 0.1% NaOH.
Ponceau
S
solution
can
be
used
to
evaluate
for
total
protein
amount
or
transfer
efficiency
on
nitrocellulose
and
PVDF
membrane.
The
PVDF
membrane
stained
Ponceau
S
for
5
mins
and
washed
with
ddH2O
for
3
mins.
It
provides
visible
pink
bands.
Lane 1,
13:
Prestained
MQ
Protein
Ladder
Lane
2
-
7:
2X
Dilutions
of
Unstained
Protein
Ladder
Lane
8
-
12:
2000,
1000,
500,
100,
50
ng
of
BSA
MQ Western Substrate Plus
The MQ Western Substrate Plus works as a luminol - based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram or high femtogram detection of antigen is enabled by MQ Western Substrate Plus shows excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film - based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
1. No
optimization
required.
Switching
to
the
MQ
Western
Substrate
Plus
from
other
brands,
such
as
Pierce
ECL
and
GE
Healthcare,
does
not
require
optimization
or
protocol
changes.
2. High
degree
of
sensitivity
and
enhanced
chemiluminescence
duration.
MQ
Western
Substrate
Plus
enables
an
accurate
low
picogram
or
high
femtogram
detection
of
protein
on
the
same
immunoblot
after
a
single
exposure.
3. Optimized
for
use
with
PVDF
and
nitrocellulose
membranes.
4. Compatible
with
Western
Blotting
Markers.
5. Optimized
for
film
-
and
CCD
-
based
imaging.
MQ Western Substrate Plus enables an accurate low picogram to high femtogram detection of protein on the same immunoblot after a single exposure. Membranes were probed with GFP tag Rabbit Poly Ab diluted at 1:10,000 of and then with Goat Anti - rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using imaging system.
MQ Western Substrate Ultra
The MQ Western Substrate Ultra works as a luminol - based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to mid femtogram detection of antigen is enabled by MQ Western Substrate Ultra shows excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film - based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
1. No
optimization
required.
Switching
to
the
MQ
Western
Substrate
Ultra
from
other
brands,
such
as
Pierce
ECL
and
GE
Healthcare,
does
not
require
optimization
or
protocol
changes.
2. High
degree
of
sensitivity
and
enhanced
chemiluminescence
duration.
MQ
Western
Substrate
Ultra
enables
an
accurate
low
picogram
or
high
femtogram
detection
of
protein
on
the
same
immunoblot
after
a
single
exposure.
3. Optimized
for
use
with
PVDF
and
nitrocellulose
membranes.
4. Compatible
with
Western
Blotting
Markers.
5. Optimized
for
film
-
and
CCD
-
based
imaging.
MQ Western Substrate Ultra enables an accurate low picogram to mid femtogram detection of protein on the same immunoblot after a single exposure. Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti - rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using imaging system.
MQ Western Substrate Plus
The MQ Western Substrate Plus works as a luminol - based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram or high femtogram detection of antigen is enabled by MQ Western Substrate Plus shows excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film - based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
1. No
optimization
required.
Switching
to
the
MQ
Western
Substrate
Plus
from
other
brands,
such
as
Pierce
ECL
and
GE
Healthcare,
does
not
require
optimization
or
protocol
changes.
2. High
degree
of
sensitivity
and
enhanced
chemiluminescence
duration.
MQ
Western
Substrate
Plus
enables
an
accurate
low
picogram
or
high
femtogram
detection
of
protein
on
the
same
immunoblot
after
a
single
exposure.
3. Optimized
for
use
with
PVDF
and
nitrocellulose
membranes.
4. Compatible
with
Western
Blotting
Markers.
5. Optimized
for
film
-
and
CCD
-
based
imaging.
MQ Western Substrate Plus enables an accurate low picogram to high femtogram detection of protein on the same immunoblot after a single exposure. Membranes were probed with GFP tag Rabbit Poly Ab diluted at 1:10,000 of and then with Goat Anti - rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using imaging system.
MQ Western Substrate Ultra
The MQ Western Substrate Ultra works as a luminol - based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to mid femtogram detection of antigen is enabled by MQ Western Substrate Ultra shows excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film - based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
1. No
optimization
required.
Switching
to
the
MQ
Western
Substrate
Ultra
from
other
brands,
such
as
Pierce
ECL
and
GE
Healthcare,
does
not
require
optimization
or
protocol
changes.
2. High
degree
of
sensitivity
and
enhanced
chemiluminescence
duration.
MQ
Western
Substrate
Ultra
enables
an
accurate
low
picogram
or
high
femtogram
detection
of
protein
on
the
same
immunoblot
after
a
single
exposure.
3. Optimized
for
use
with
PVDF
and
nitrocellulose
membranes.
4. Compatible
with
Western
Blotting
Markers.
5. Optimized
for
film
-
and
CCD
-
based
imaging.
MQ Western Substrate Ultra enables an accurate low picogram to mid femtogram detection of protein on the same immunoblot after a single exposure. Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti - rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using imaging system.
Phosphate Buffered Saline (1X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 1X Working Solution. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Phosphate Buffered Saline (10X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 10X concentrate. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
To make one liter of 1X working concentration of PBS, mix 100ml of 10X Phosphate B uffered Saline in 900ml of sterile distilled water. In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Phosphate Buffered Saline (1X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 1X Working Solution. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Phosphate Buffered Saline (10X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 10X concentrate. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
To make one liter of 1X working concentration of PBS, mix 100ml of 10X Phosphate B uffered Saline in 900ml of sterile distilled water. In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Easy Protein Gel
MOLEQULE - ON offers pre - cast polyacrylamide gels that are ready to run and designed to give optimal separation of small to medium - sized proteins. Easy protein gels provide high quality and reliability and eliminate exposure to toxic unpolymerized acrylamide. Use Easy Protein Gels for preparing proteins for SDS - PAGE, native PAGE, peptide separations, nucleic acid separations, western blotting, sequencing, mass spectrometry, and any other techniques where protein integrity is required. A pack of running buffer is included in Easy Protein Gel package (one box of 10 gels).
1. No.
of
wells:
10
wells
2. Outer
gel
dimension:
8cm
x
10
cm
3. Thickness
of
gel
layer:
1.5
mm
4. Maximum
loading
volume
of
gel
well:
25 μl
5. Gradient:
Tris
HEPES
Gradient
(4
-
20
%)
Easy Protein Gel is
suitable for the most of mini SDS
-
PAGE electrophoresis tank,
including
1. Bio
-
Rad Mini
-
PROTEAN (II/3 /Tetra System)
2. Hoefer
Mighty Small (SE 250/ SE 260/ SE 280)
3. Life
Technology
Novex
Mini
-
Cell (use with special baffle)
MQ Western One Step Blocker
MQ Western One Step Blocker is a blocking solution for Western blot analysis. This Blocker buffer not only provides blocking and primary and secondary antibody hybridization in one step but also enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. It, therefore, serves as both blocker and enhancer in Western analysis. With the three - in - one step procedure, MQ Western One Step Blocker is a time and labor economic solution for the time consuming and laborious Western procedure.
1°Ab
(ab
8227,
abcam
):
Rabbit
anti
beta
actin;
1:5,000
2°Ab
(ab
205718,
abcam
):
Goat
anti
Rabbit
HRP;
1:5,000
MQ Western Stripping Buffer
Nitrocellulo se and PVDF membranes probed by Western blotting procedures and detected by chemiluminescent or other non - precipitating substrates can be stripped and re - probed using MQ Western Stripping Buffer. Western blotting is widely used to detect and compare proteins in complex mixtures, and chemiluminescence has largely replaced chromogenic substrates as the most convenient and sensitive method of detection. One advantage of chemiluminescence is the ability to strip and re - probe the protein mixture on the membrane. Traditional stripping methods use conditions that are effective for only low - affinity antibody - antigen interactions or are so harsh that they tend to adversely alter the antigen for subsequent immunoprobing. MQ Western Stripping Buffer is a robust but gentle formulation for stripping primary and secondary antibodies from blots to enable several reprobings on the same membrane.
Tris Buffered Saline Buffer, TBS, (20X)
Tris Buffered Saline (TBS) is a buffering solution that stabilizes pH. The solution is commonly used for western blot and ELISA procedures. It is used as a convenient prepared buffer for use in various applications.
To make one liter of 1X working concentration, take 50ml of 20X TBS Buffer and mix with 950ml of sterile distilled water. 1X Buffer contains 0.14M Sodium Chloride, 3mM Potassium Chloride and 25mM Tris Base.
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Lorem ipsum dolor sit amet, consectetur adipisicing elit. Optio, neque qui velit. Magni dolorum quidem ipsam eligendi, totam, facilis laudantium cum accusamus ullam voluptatibus commodi numquam, error, est. Ea, consequatur.
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Optio, neque qui velit. Magni dolorum quidem ipsam eligendi, totam, facilis laudantium cum accusamus ullam voluptatibus commodi numquam, error, est. Ea, consequatur.
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Optio, neque qui velit. Magni dolorum quidem ipsam eligendi, totam, facilis laudantium cum accusamus ullam voluptatibus commodi numquam, error, est. Ea, consequatur.
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Optio, neque qui velit. Magni dolorum quidem ipsam eligendi, totam, facilis laudantium cum accusamus ullam voluptatibus commodi numquam, error, est. Ea, consequatur.
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Optio, neque qui velit. Magni dolorum quidem ipsam eligendi, totam, facilis laudantium cum accusamus ullam voluptatibus commodi numquam, error, est. Ea, consequatur.
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Optio, neque qui velit. Magni dolorum quidem ipsam eligendi, totam, facilis laudantium cum accusamus ullam voluptatibus commodi numquam, error, est. Ea, consequatur.
Lorem ipsum dolor sit amet, consectetur adipisicing elit. Optio, neque qui velit. Magni dolorum quidem ipsam eligendi, totam, facilis laudantium cum accusamus ullam voluptatibus commodi numquam, error, est. Ea, consequatur.