The MQ Blood RNA Extraction Kit is based on a simple spin column technique to get high quality, high-purity intact total RNA. The reagent contains disruptive and protective properties of guanidine isothiocyanate and β-mercapto-ethanol to inactivate the ribonucleases present in cell extracts. RNA in the whole homogeneity is selectively absorbed on the spin column, and other impurities are washed away. Total RNA is eluted from the membrane in the presence of RNase-free water. 5-15 μg total RNA can be purified from 200 μl of anticoagulated blood sample using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly (A) selection, and in vitro translation.

MQ Tissue RNA Extraction Kit allows efficient extraction of total RNA from various samples. Total RNA is easily purified from animal or human cells and tissues using a simple spin format. This kit simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-based purification. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide optimal binding conditions. The lysate is then loaded onto the MQ column with a silica membrane. While the RNA binds to the silica membrane, all proteins and other components are removed in the flow-through. Remaining contaminants and salts are efficiently washed away. The purified RNA eluted in RNase-free water has OD 260/280 ratios of 1.9-2.1 (measured in 10 mM Tris HCl, pH 7.5) and is ideal for use in most downstream applications including Northern blotting, RT-PCR, Quantitative PCR, Poly (A) RNA selection, and Array analysis.

MQ Tissue RNA Extraction Kit (with gDNA Eliminator Column) is designed to purify RNA from small amounts of animal cells or tissues. Samples are lysed and homogenized by Buffer Lysis DR. Genomic DNA contamination is effectively removed using a specially gDNA Eliminator Column. RNA is absorbed on MQ RNA Column. Finally, the RNA is eluted from MQ RNA Column. The purified RNA is ready to use and is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as quantitative, real-time RT-PCR. The procedure is simple and fast, requires no need for phenol/chloroform extraction. The whole procedure takes less than 30 minutes.

MQ microRNA Extraction Kit is used for the isolation of miRNA, siRNA, snRNA (< 200 nt) and total RNA. This miRNA Extractor performs well with both small and large quantities of tissue and cells from human, animal, plant, or bacterial origin. The kit provides a rapid and efficient method for purifying and enriching miRNA along with other small RNAs, allowing researchers to obtain high-quality miRNA directly from cells or tissues as rapidly and simply as a total RNA prep.

MQ Fungal RNA extraction Kit is designed for preparation of high-quality total RNA from a wide range of fungal species. Fungal samples are lysed and homogenized by Buffer Rlysis FG. RNA in the whole homogeneity is selectively absorbed on a spin column, and other impurities are washed away. Total RNA is eluted from the membrane in the presence of RNase-Free Water in the final step. 3-5 μg total RNA can be purified from 30 mg filamentous fungi using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection, and in vitro translation.

Polysaccharides and polyphenols are components of plants, it is very difficult to remove after they form insoluble compounds closely combining with RNA. MQ Plant RNA Extraction Kit is applicable to all kinds of plant samples for RNA rapid extraction. Cracking liquid can effectively solve difficult problems such as polyphenols' easy oxidation, polysaccharide separation, and nucleic acids compounds. RNA purification using a spin column is easy to operate, avoiding ethanol rinse. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection, and in vitro translation.

MQ Viral RNA Extraction Kit simplifies isolation of viral RNA from cell-free body fluids with fast spin-column format. No phenol/chloroform extraction is required. Viral RNA binds specifically to the silica membrane while contaminants are removed in the flow-through. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in RNase-free Water. Purified RNA is ready to use in RT-PCR, Northern blotting, or other downstream applications.

RNase Inhibitor offered by MOLEQULE-ON is a highly purified, nuclease-free preparation. It is supplied at a concentration of 40,000 units/ml or 40 units/μl, in a buffer containing 20 mM HEPES-KOH (pH 7.6), 50 mM KCl, and 8 mM DTT. Each lot is rigorously tested and is guaranteed free of non-specific endonuclease, exonuclease, and RNase activity.

It does not inhibit RNA polymerases, DNA polymerases, RNase H, or RNase T1, and is therefore ideal for use in RT-PCR reactions, in vitro transcription, or cDNA library construction. One unit of this protein will inhibit 50% of the activity of 5 ng RNase A.

It is used to avoid RNase contamination. Add inhibitor to achieve a final concentration of 1 unit/μl in the reaction. During assembly of a reaction, RNase Inhibitor should be added before other components that are a possible source of RNase contamination (i.e. enzymes, plasmid from a mini-prep).