MOLEQULE-ON reagents and kits are essential tool in research and various field of science, which enable accurate experiments and tests. Our products are integrated with newest technologies to meet the demand of a wide range of laboratories. The list includes PCR and qPCR reagents, electrophoresis, enzymes, nucleic acid extraction and purification kits, colorimetric and ELISA kits and buffers.
MQ cDNA Synthesis Kit
MQ cDNA Synthesis Kit is engineered innovatively and specifically for Research and Laboratory applications for meeting all your cDNA synthesis needs and for overcoming the most challenging secondary RNA structures over a wide temperature range . The Kit contains our next - generation, engineered recombinant M - MLV reverse transcriptase, with improved thermostability, processivity, robustness, optimal cDNA yields, proprietary site mutations for reduced RNase H activity, and extended half - life, is the most versatile reverse transcriptase in the world for not only simply meeting the routine cDNA synthesis requirements but also enabling superior performance for even the most challenging RNA samples at hand .
The stability RT - qPCR data shows the performance of MQ RScript reverse transcriptase is maintained without any significant alteration in performance at 37 ° C after a period of 3 weeks ( 21 days), demonstrating its high resistance to temperature and time . The percentage of MQ RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature.
To evaluate the RT performance for the COVID - 19 fragments, the N1 gene with 100 copies per reaction was tested in the one - step RT - qPCR. Experiments proved to be feasible and effective (mean Ct : 34.88).
A: MQ
Rscript
Reverse Transcriptase Cat# EN
-
M
-
005
-
5000
B:
Competitor 1
C: Competitor
2
D: Competitor
3
Reverse transcription of a 1 kb fragment ARHGAP 29 RNA using MQ RScript reverse transcriptase or competitor reverse transcriptases were used to carry this experiment . Regardless of the variations in temperatures ( 42 - 60 ° C) MQ RScript reverse transcriptase shows a stable and superior performance at higher temperatures of up to 60 °C compared to other brands . The molecular weight marker used was DNA Ladder 1 kb.
MQ RScript reverse transcriptase was used in a reaction with a range of human bladder cancer cell lines - 5637 (HTB - 9) RNA. The resulting synthesized cDNA (8 kb ,11kb) was followed by PCR and visualized on a 2% agarose gel. The molecular weight marker used was DNA Ladder 1kb.
At a temperature of 55 °C, MQ RScript Reverse Transcriptase exceeds performance demonstrating better efficiency and higher cDNA yields. MQ RScript Reverse Transcriptase shows a stable and superior performance at 55 ℃ compared to other brands. Therefore MQ RScript reverse transcriptase does not encounter significant higher Ct values compared to other brands due to their low amounts of input cDNA.
Red: MQ
RScript
Reverse Transcriptase #EN
-
M
-
005
-
5000
Green:
Competitor 1
Blue:
Competitor 2
Hi - Tech Green 2X qPCR Universal Mix
Hi - Tech Green 2 X qPCR Universal Master Mix is a 2 X concentrated, ready for use Master Mix reaction enhanced for dye - based quantitative PCR (qPCR) and compatible with the majority of commercially available real - time PCR systems (ROX - independent and ROX - dependent) . It contains NanoTaq hot - start DNA polymerase, dNTPs, MgCl₂ , Hi - Tech Green I dye, enhancers, stabilizers and essentials for a success PCR reaction .
Hi - Tech Green qPCR Mix is ideally suited for :
A 500 bp human genomic DNA target was used to compare the two leading competing qPCR Master Mixes. All amplifications were performed in accordance with the manufacturer’s instructions. Hi-Tech Green 2X qPCR Universal Mix exhibited to be effective (mean Ct : 21.29) and highly specific because no second amplification signal could be identified with the melting curve. The results show that it has the best performance as compared with other qPCR Master Mix suppliers.
MQ One Step Hi - Tech Green 2X qPCR Ready Mix
MQ One Step Hi - Tech Green 2 X qPCR Ready Mix delivers high sensitivity of the target RNA level due to its MQ engineered reverse transcriptase, a reduced RNase H+ activity MMLV enzyme in addition to a powerful RNase inhibitors mix which aim to diminish RNA degradation and mispriming during reaction setup and reverse transcription to guarantee optimal RT efficiency .
MQ One Step Green qPCR Mix is ideally suited for :
The VP 1 capsid gene of EV 71 virus with serial diluted RNA template concentrations are prepared and conducted by qPCR . Based on the detected SYBR Green fluorescence values, the result shows the Ct values corresponding to the different concentration.
Please verify the specificity of the primer pair by blasting the template’s organism (Primer - BLAST : http : // www . ncbi . nlm . nih . gov /tools/primer - blast/) . The primers should amplify an amplicon with 80 – 200 bp . Do not exceed 400 bp . Extension and annealing time can reduced by amplification of smaller amplicons . Using the default settings of primer 3 software , the melting temperature should be 60°C.
MQ One Step Probe 2X qPCR Ready Mix
MQ OneStep probe qPCR Mix delivers high sensitivity of the target RNA level due to its unique reverse transcriptase, a reduced RNase H+ activity MMLV enzyme in addition to a powerful RNase inhibitors mix which aim to diminish RNA degradation and mispriming during reaction setup and reverse transcription to guarantee optimal RT efficiency .
MQ One Step Green qPCR Mix is ideally suited for :
Please verify the specificity of the primer pair by blasting the template’s organism (Primer - BLAST : http : // www . ncbi . nlm . nih . gov /tools/primer - blast/) . The primers should amplify an amplicon with 80 – 200 bp . Do not exceed 400 bp . Extension and annealing time can reduced by amplification of smaller amplicons . Using the default settings of primer 3 software , the melting temperature should be 60 ° C.
To evaluate the performance, the Ribonuclease P (RP ) gene with varying RNA template concentrations, through serial dilutions, were used . Based on the fluorescence value of the RP probe, the results show the performance corresponding to the Ct value with different copy numbers.
MQ One Step Probe 2X qPCR Ready Mix
MQ OneStep probe qPCR Mix delivers high sensitivity of the target RNA level due to its unique reverse transcriptase, a reduced RNase H+ activity MMLV enzyme in addition to a powerful RNase inhibitors mix which aim to diminish RNA degradation and mispriming during reaction setup and reverse transcription to guarantee optimal RT efficiency .
MQ One Step Green qPCR Mix is ideally suited for :
Please verify the specificity of the primer pair by blasting the template’s organism (Primer - BLAST : http : // www . ncbi . nlm . nih . gov /tools/primer - blast/) . The primers should amplify an amplicon with 80 – 200 bp . Do not exceed 400 bp . Extension and annealing time can reduced by amplification of smaller amplicons . Using the default settings of primer 3 software , the melting temperature should be 60 ° C.
To evaluate the performance, the Ribonuclease P (RP ) gene with varying RNA template concentrations, through serial dilutions, were used . Based on the fluorescence value of the RP probe, the results show the performance corresponding to the Ct value with different copy numbers.
MQ Reverse Transcriptase
MQ Rscript Reverse Transcriptase is engineered innovatively and specifically for Research and Laboratory applications for meeting all your cDNA synthesis needs and for overcoming the most challenging secondary RNA structures over a wide temperature range . The Kit contains our next - generation, engineered recombinant M - MLV reverse transcriptase, with improved thermostability, processivity, robustness, optimal cDNA yields, proprietary site mutations for reduced RNase H activity, and extended half - life, is the most versatile reverse transcriptase in the world for not only simply meeting the routine cDNA synthesis requirements but also enabling superior performance for even the most challenging RNA samples at hand.
The stability RT - qPCR data shows the performance of MQ RScript reverse transcriptase is maintained without any significant alteration in performance at 37 ° C after a period of 3 weeks ( 21 days), demonstrating its high resistance to temperature and time . The percentage of MQ RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature.
To evaluate the RT performance for the COVID - 19 fragments, the N1 gene with 100 copies per reaction was tested in the one - step RT - qPCR. Experiments proved to be feasible and effective (mean Ct : 34.88).
Reverse transcription of a 1 kb fragment ARHGAP 29 RNA using MQ RScript reverse transcriptase or competitor reverse transcriptases were used to carry this experiment . Regardless of the variations in temperatures ( 42 - 60 ° C) MQ RScript reverse transcriptase shows a stable and superior performance at higher temperatures of up to 60 °C compared to other brands . The molecular weight marker used was DNA Ladder 1 kb.
MQ RScript reverse transcriptase was used in a reaction with a range of human bladder cancer cell lines - 5637 (HTB - 9) RNA. The resulting synthesized cDNA (8 kb ,11kb) was followed by PCR and visualized on a 2% agarose gel. The molecular weight marker used was DNA Ladder 1kb
At a temperature of 55 ° C, MQ RScript Reverse Transcriptase exceeds performance demonstrating better efficiency and higher cDNA yields . MQ RScript Reverse Transcriptase shows a stable and superior performance at 55 °C compared to other brands . Therefore MQ RScript reverse transcriptase does not encounter significant higher Ct values compared to other brands due to their low amounts of input cDNA .
ROX Reference Dye
6 - carboxyl - X - Rhodamine common ly known as ROX dye is a passive reference dye for qPCR applications. It is an inert fluorescent dye that can be added as one of the components in a qPCR master mix . High ROX is usually 500nM and Low ROX is 50nM . The ROX concentration depends on the q PCR instrument or, more precisely, on the filter available. ROX compensate for well to well variations in qPCR reaction . Variations can occur due variation in light i ntensity, which depends on the optical construction of the PCR machine or due to pipetting error . The ROX fluorescence does not change during the PCR reaction but provide a stable baseline to which samples are normalized. The excitation and emission of the reference dye are 584 nm and 612nm, respectively.
MQ cDNA Synthesis Kit
MQ cDNA Synthesis Kit is engineered innovatively and specifically for Research and Laboratory applications for meeting all your cDNA synthesis needs and for overcoming the most challenging secondary RNA structures over a wide temperature range . The Kit contains our next - generation, engineered recombinant M - MLV reverse transcriptase, with improved thermostability, processivity, robustness, optimal cDNA yields, proprietary site mutations for reduced RNase H activity, and extended half - life, is the most versatile reverse transcriptase in the world for not only simply meeting the routine cDNA synthesis requirements but also enabling superior performance for even the most challenging RNA samples at hand .
The stability RT - qPCR data shows the performance of MQ RScript reverse transcriptase is maintained without any significant alteration in performance at 37 ° C after a period of 3 weeks ( 21 days), demonstrating its high resistance to temperature and time . The percentage of MQ RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature.
To evaluate the RT performance for the COVID - 19 fragments, the N1 gene with 100 copies per reaction was tested in the one - step RT - qPCR. Experiments proved to be feasible and effective (mean Ct : 34.88).
A: MQ
Rscript
Reverse Transcriptase Cat# EN
-
M
-
005
-
5000
B:
Competitor 1
C: Competitor
2
D: Competitor
3
Reverse transcription of a 1 kb fragment ARHGAP 29 RNA using MQ RScript reverse transcriptase or competitor reverse transcriptases were used to carry this experiment . Regardless of the variations in temperatures ( 42 - 60 ° C) MQ RScript reverse transcriptase shows a stable and superior performance at higher temperatures of up to 60 °C compared to other brands . The molecular weight marker used was DNA Ladder 1 kb.
MQ RScript reverse transcriptase was used in a reaction with a range of human bladder cancer cell lines - 5637 (HTB - 9) RNA. The resulting synthesized cDNA (8 kb ,11kb) was followed by PCR and visualized on a 2% agarose gel. The molecular weight marker used was DNA Ladder 1kb.
At a temperature of 55 °C, MQ RScript Reverse Transcriptase exceeds performance demonstrating better efficiency and higher cDNA yields. MQ RScript Reverse Transcriptase shows a stable and superior performance at 55 ℃ compared to other brands. Therefore MQ RScript reverse transcriptase does not encounter significant higher Ct values compared to other brands due to their low amounts of input cDNA.
Red: MQ
RScript
Reverse Transcriptase #EN
-
M
-
005
-
5000
Green:
Competitor 1
Blue:
Competitor 2
Hi - Tech Green 2X qPCR Universal Mix
Hi - Tech Green 2 X qPCR Universal Master Mix is a 2 X concentrated, ready for use Master Mix reaction enhanced for dye - based quantitative PCR (qPCR) and compatible with the majority of commercially available real - time PCR systems (ROX - independent and ROX - dependent) . It contains NanoTaq hot - start DNA polymerase, dNTPs, MgCl₂ , Hi - Tech Green I dye, enhancers, stabilizers and essentials for a success PCR reaction .
Hi - Tech Green qPCR Mix is ideally suited for :
A 500 bp human genomic DNA target was used to compare the two leading competing qPCR Master Mixes. All amplifications were performed in accordance with the manufacturer’s instructions. Hi-Tech Green 2X qPCR Universal Mix exhibited to be effective (mean Ct : 21.29) and highly specific because no second amplification signal could be identified with the melting curve. The results show that it has the best performance as compared with other qPCR Master Mix suppliers.
MQ One Step Hi - Tech Green 2X qPCR Ready Mix
MQ One Step Hi - Tech Green 2 X qPCR Ready Mix delivers high sensitivity of the target RNA level due to its MQ engineered reverse transcriptase, a reduced RNase H+ activity MMLV enzyme in addition to a powerful RNase inhibitors mix which aim to diminish RNA degradation and mispriming during reaction setup and reverse transcription to guarantee optimal RT efficiency .
MQ One Step Green qPCR Mix is ideally suited for :
The VP 1 capsid gene of EV 71 virus with serial diluted RNA template concentrations are prepared and conducted by qPCR . Based on the detected SYBR Green fluorescence values, the result shows the Ct values corresponding to the different concentration.
Please verify the specificity of the primer pair by blasting the template’s organism (Primer - BLAST : http : // www . ncbi . nlm . nih . gov /tools/primer - blast/) . The primers should amplify an amplicon with 80 – 200 bp . Do not exceed 400 bp . Extension and annealing time can reduced by amplification of smaller amplicons . Using the default settings of primer 3 software , the melting temperature should be 60°C.
MQ Probe 2X qPCR Universal Mix
MQ Probe 2 X qPCR Universal Mix is a 2 X concentrated, ready - to - use master mix optimized for probe - based real - time PCR and compatible with the majority of commercially available real - time PCR systems (ROX - independent and ROX - dependent) . It contains antibody - mediated hot - start Taq DNA polymerase, dNTPs , MgCl₂ , enhancers, stabilizers and essentials for a success PCR reaction .
MQ Probe qPCR Mix is ideally suited for :
Please verify the specificity of the primer pair by blasting the template’s organism (Primer - BLAST : http : // www . ncbi . nlm . nih . gov /tools/primer - blast/) . The primers should amplify an amplicon with 80 – 200 bp . Do not exceed 400 bp . Extension and annealing time can reduced by amplification of smaller amplicons . Using the default settings of primer 3 software , the melting temperature should be 60 ° C .
The SARS - CoV - 2 nucleocapsid (N 1 ) gene with serial diluted DNA template concentrations are prepared and conducted by qPCR . Based on the detected N 1 probe fluorescence values, the result shows the Ct values corresponding to the different concentration.
MQ One Step Probe 2X qPCR Ready Mix
MQ OneStep probe qPCR Mix delivers high sensitivity of the target RNA level due to its unique reverse transcriptase, a reduced RNase H+ activity MMLV enzyme in addition to a powerful RNase inhibitors mix which aim to diminish RNA degradation and mispriming during reaction setup and reverse transcription to guarantee optimal RT efficiency .
MQ One Step Green qPCR Mix is ideally suited for :
Please verify the specificity of the primer pair by blasting the template’s organism (Primer - BLAST : http : // www . ncbi . nlm . nih . gov /tools/primer - blast/) . The primers should amplify an amplicon with 80 – 200 bp . Do not exceed 400 bp . Extension and annealing time can reduced by amplification of smaller amplicons . Using the default settings of primer 3 software , the melting temperature should be 60 ° C.
To evaluate the performance, the Ribonuclease P (RP ) gene with varying RNA template concentrations, through serial dilutions, were used . Based on the fluorescence value of the RP probe, the results show the performance corresponding to the Ct value with different copy numbers.
MQ Reverse Transcriptase
MQ Rscript Reverse Transcriptase is engineered innovatively and specifically for Research and Laboratory applications for meeting all your cDNA synthesis needs and for overcoming the most challenging secondary RNA structures over a wide temperature range . The Kit contains our next - generation, engineered recombinant M - MLV reverse transcriptase, with improved thermostability, processivity, robustness, optimal cDNA yields, proprietary site mutations for reduced RNase H activity, and extended half - life, is the most versatile reverse transcriptase in the world for not only simply meeting the routine cDNA synthesis requirements but also enabling superior performance for even the most challenging RNA samples at hand.
The stability RT - qPCR data shows the performance of MQ RScript reverse transcriptase is maintained without any significant alteration in performance at 37 ° C after a period of 3 weeks ( 21 days), demonstrating its high resistance to temperature and time . The percentage of MQ RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature.
To evaluate the RT performance for the COVID - 19 fragments, the N1 gene with 100 copies per reaction was tested in the one - step RT - qPCR. Experiments proved to be feasible and effective (mean Ct : 34.88).
Reverse transcription of a 1 kb fragment ARHGAP 29 RNA using MQ RScript reverse transcriptase or competitor reverse transcriptases were used to carry this experiment . Regardless of the variations in temperatures ( 42 - 60 ° C) MQ RScript reverse transcriptase shows a stable and superior performance at higher temperatures of up to 60 °C compared to other brands . The molecular weight marker used was DNA Ladder 1 kb.
MQ RScript reverse transcriptase was used in a reaction with a range of human bladder cancer cell lines - 5637 (HTB - 9) RNA. The resulting synthesized cDNA (8 kb ,11kb) was followed by PCR and visualized on a 2% agarose gel. The molecular weight marker used was DNA Ladder 1kb
At a temperature of 55 ° C, MQ RScript Reverse Transcriptase exceeds performance demonstrating better efficiency and higher cDNA yields . MQ RScript Reverse Transcriptase shows a stable and superior performance at 55 °C compared to other brands . Therefore MQ RScript reverse transcriptase does not encounter significant higher Ct values compared to other brands due to their low amounts of input cDNA .
ROX Reference Dye
6 - carboxyl - X - Rhodamine common ly known as ROX dye is a passive reference dye for qPCR applications. It is an inert fluorescent dye that can be added as one of the components in a qPCR master mix . High ROX is usually 500nM and Low ROX is 50nM . The ROX concentration depends on the q PCR instrument or, more precisely, on the filter available. ROX compensate for well to well variations in qPCR reaction . Variations can occur due variation in light i ntensity, which depends on the optical construction of the PCR machine or due to pipetting error . The ROX fluorescence does not change during the PCR reaction but provide a stable baseline to which samples are normalized. The excitation and emission of the reference dye are 584 nm and 612nm, respectively.
