MOLEQULE-ON reagents and kits are essential tool in research and various field of science, which enable accurate experiments and tests. Our products are integrated with newest technologies to meet the demand of a wide range of laboratories. The list includes PCR and qPCR reagents, electrophoresis, enzymes, nucleic acid extraction and purification kits, colorimetric and ELISA kits and buffers.
PCR reagents, dehydrated culture media and plasticware. Our company is focused on customer satisfaction striving to
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MQ Blood Genomic DNA Extraction Kit
MQ Blood Genomic DNA Extraction Kit with Spin Columns provides a fast, simple, and efficient method for the purification of genomic DNA from blood cells. The kit uses silica-based DNA purification technology, where DNA is selectively adsorbed onto the silica-based membrane embedded in the MQ Spin Column. Other components and impurities flow through the column, and the genomic DNA is then eluted off the column, making it ready for use in most downstream applications. Importantly, this method does not require hazardous compounds such as phenol, chloroform, or CsCl are not required.
1. MQ Blood DNA Extraction Kit procedure is simple, fast, and efficient.
2. It prepared high-quality DNA that can be used in any downstream applications such as sequencing, PCR, cloning, transformation, or restriction digestions.
3. The protocol is reproducible and it gives a high yield of DNA, up to 10 μg of DNA per column.
MQ Blood DNA Isolation Kit utilizes a silica-gel membrane that selectively absorbs up to 10 μg of DNA fragments in the presence of specialized binding buffers. Nucleotides, oligos (less than 40 bases), enzymes, mineral oil, and other impurities do not bind to the membrane and are washed away. The DNA fragments can then be eluted off the column in a small volume and used in downstream applications without further processing.
MQ Blood Genomic DNA Extraction Kit
MQ Blood Genomic DNA Extraction Kit uses a centrifugal adsorption column that can specifically bind DNA and a unique buffer system to extract genomic DNA from whole blood. The silicon matrix material used in the centrifugal adsorption column is a unique new material, which can efficiently and specifically adsorb DNA, and can remove impurity proteins and other organic compounds in cells to the greatest extent. The extracted genomic DNA fragments are large, high in purity, stable, and reliable in quality. The genomic DNA extracted by this kit can be used in various routine operations, including restriction enzyme digestion, PCR, library construction, Southern hybridization, and other experiments.
1. Fast and easy processing using a rapid spin-column format. The entire procedure takes approximately 30 minutes.
2. High quality of DNA. OD 260/280 of purified DNA is generally 1.7-1.9.
3. No phenol/chloroform extraction or ethanol precipitation is required.
MQ Tissue Genomic DNA Extraction Kit
MQ Tissue Genomic DNA Isolation Kit is designed for rapid purification of genomic DNA (as well as other forms of DNA, e.g., mitochondrial DNA) from fresh, frozen, or paraffin-embedded animal tissues and cells.
Samples are first lysed using proteinase K in an optimized buffer. The lysate is loaded onto the MQ spin column, where DNA is selectively bound to the EX membrane embedded in the column. During wash steps, protein and other impurities are removed, and DNA is then eluted in a low-salt buffer. Purified DNA typically has A₂₆₀/A₂₈₀ ratios of 1.7-1.9 and is highly suited for most downstream applications such as PCR, Southern blotting, RAPD, and RFLP.
The purification procedure requires no phenol/chloroform extraction or alcohol precipitation and involves minimal handling. The entire procedure takes only 20 minutes after sample preparation. Yields of DNA vary between samples depending on the amount and type of tissue; for most tissues, 5-10 μg of DNA can be obtained from 25 mg of materials.
1. High quality of DNA, OD 260/OD 280 of purified DNA is generally 1.7~1.9.
2. Purification of high quality genomic DNA with a molecular weight ≥ 20 kb.
3. Fast and effective. Fast and easy processing using a rapid spin-column format.
4. Compatible with many downstream applications such as PCR, restriction digestion, and hybridization.
5. No phenol/chloroform extraction or ethanol precipitation is required. High yield and reproductivity.
6. The kit is fit for a variety of sample sources.
MQ Genomic DNA Extraction Kit
MQ Genomic DNA Isolation Kit is designed for rapid purification of genomic DNA (as well as other forms of DNA, e.g. mitochondria) from fresh, frozen or paraffin-embedded animal tissues, cells, blood, or bacteria.
Samples are firstly lysed using proteinase K in optimized buffer. The lysate is loaded onto the MQ spin column, and DNA is selectively bound to EX membrane embedded in the column. During wash steps, protein and other impurities are removed and DNA is then eluted in low-salt buffer. Purified DNA typically has an A₂₆₀/A₂₈₀ ratio of 1.7-1.9, and is highly suited for most downstream applications such as PCR, Southern blotting, RAPD, and RFLP.
The purification procedure requires no phenol/chloroform extraction or alcohol precipitation, and involves minimal handling. The whole procedure takes only 20 minutes after sample preparation.
Yields of DNA vary between samples depending on the amount and type of tissue; for most tissues, 5-10 μg DNA can be obtained from 25 mg of materials.
1. High quality of DNA, OD 260/OD 280 of purified DNA is generally 1.7~1.9.
2. Purification of high quality genomic DNA with a molecular weight ≥ 20 kb.
3. Fast and effective. Fast and easy processing using a rapid spin-column format.
4. Compatible with many downstream applications such as PCR, restriction digestion, and hybridization.
5. No phenol/chloroform extraction or ethanol precipitation is required. High yield and reproductivity.
6. The kit is fit for a variety of sample sources.
MQ Plant Genomic DNA Extraction Kit
MQ Plant Genomic DNA Extraction Kit provides a simple and convenient technique to isolate high quality DNA from plants using a rapid spin-column format. DNA of cell lysates is selectively bound to the spin column and other impurities such as proteins and salts do not bind to the column and are eliminated in flow through. No phenol extraction and ethanol precipitations are required. The kit is also suitable for isolation of bacterial genomic DNA from colonies on dish. Purified genomic DNA is 20-50 kb in length. Purified DNA is suitable for downstream applications such as Restriction Endonuclease Digestions, PCR, and other applications.
1. Fast and easy. Processing uses a rapid spin-column format. The entire procedure takes approx. 30 minutes.
2. Versatile. Various plant species have been tested.
3. High quality of DNA. OD₂₆₀/OD₂₈₀ of purified DNA is generally 1.7-1.9.
4. The purified DNA is ready-to-use for most downstream applications.
MQ Fungal Genomic DNA Extraction Kit
The kit provides a simple and convenient technique to isolate high quality DNA from Fungi using a rapid spin-column format. DNA of cell lysate is selectively bound to the spin column and other impurities such as proteins, salts do not bind on the column and are eliminated in flow through. No phenol extraction, no ethanol precipitations are required. Purified genomic DNA ranges 20-50 kb in length can be obtained. Purified DNA is suitable for downstream applications such as Restriction Endonuclease Digestions, PCR, and other applications.
1. MQ Fungal Genomic DNA Extraction Kit procedure is simple, fast, and efficient.
2. It prepares high quality DNA that can be used in any downstream applications such as sequencing, PCR, cloning, transformation, or restriction digestions.
3. The protocol is reproducible.
4. It gives high yield of DNA, up to 10 μg of DNA per column.
MQ Yeast Genomic DNA Extraction Kit
MQ Yeast Genomic DNA Extraction Kit provides a simple and convenient technique to isolate high quality DNA from yeast using a rapid spin-column format. DNA of cell lysate is selectively bound to the spin column and other impurities such as proteins, salts do not bind on the column and are eliminated in flow through. No phenol extraction, no ethanol precipitations are required. The kit is also suitable for isolation of yeast genomic DNA from colonies on dish. Purified genomic DNA is 20-50 kb in length. Purified DNA is suitable for downstream applications such as Restriction Endonuclease Digestions, PCR, and other applications.
1. Fast and easy processing using a rapid spin-column format. The entire procedure takes approximately 30 minutes.
2. High yield. 5-20 μg of yeast genomic DNA can be obtained from 1 ml (10⁸-10⁹ cells) of overnight culture.
3. High quality of DNA. OD 260/280 of purified DNA is generally 1.7-1.9.
4. No phenol/chloroform extraction or ethanol precipitation is required.
MQ Bacterial DNA Extraction Kit
MQ Bacterial DNA Extraction Kit provides a simple and convenient technique to isolate high quality DNA from both Gram negative and Gram positive bacteria using a rapid spin-column format. DNA of cell lysate is selectively bound to the spin column and other impurities such as proteins, salts do not bind on the column and are eliminated in flow through. No phenol extraction, no ethanol precipitations are required. The kit is also suitable for isolation of bacterial genomic DNA from colonies on dish. Purified genomic DNA can be up to 50 kb in length. Purified DNA is suitable for downstream applications such as Restriction Endonuclease Digestions, PCR, and other applications.
1. Fast and easy processing using a rapid spin-column format. The entire procedure takes approximately 30 minutes.
2. High yield. 5-20 μg of yeast genomic DNA can be obtained from 1 ml (10⁸-10⁹ cells) of overnight culture.
3. High quality of DNA. OD 260/280 of purified DNA is generally 1.7-1.9.
4. No phenol/chloroform extraction or ethanol precipitation is required.
MQ Bacterial Genomic DNA Extraction Kit
The kit provides a simple and convenient technique to isolate high quality DNA from both Gram negative and Gram positive bacteria using a rapid spin-column format. DNA of cell lysate is selectively bound to the spin column and other impurities such as proteins, salts do not bind on the column and are eliminated in flow through. No phenol extraction, no ethanol precipitations are required. The kit is also suitable for isolation of bacterial genomic DNA from colonies on dish. Purified genomic DNA can be up to 50 kb in length. Purified DNA is suitable for downstream applications such as Restriction Endonuclease Digestions, PCR, and other applications.
1. Fast and easy processing using a rapid spin-column format. The entire procedure takes approximately 30 minutes.
2. High yield. 5-20 μg of yeast genomic DNA can be obtained from 1 ml (10⁸-10⁹ cells) of overnight culture.
3. High quality of DNA. OD 260/280 of purified DNA is generally 1.7-1.9.
4. No phenol/chloroform extraction or ethanol precipitation is required.
MQ Soil DNA Extraction Kit
MQ Soil DNA Extraction Kit is designed for the preparation of high-quality DNA from sand, soil, and fecal samples. These samples are considered challenging as they contain rich humic acid that may interfere with PCR reactions. The kit provides simple, rapid isolation of PCR-ready total DNA from soil. Purified DNA does not contain humic acid and can be used for PCR and other downstream applications. The molecular size of the purified DNA is around 20-50 kb. Average DNA yields are 5-50 μg per gram of the soil sample.
1. Fast isolation of high-quality DNA from sand, soil and fecal samples.
MQ Plasmid DNA Mini Extraction Kit
The MQ Plasmid Extraction system provides a fast, simple, and cost-effective plasmid miniprep method for routine molecular biology laboratory applications. This kit uses a modified alkaline lysis method and silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Phenol extraction and ethanol precipitation are not required. It reduces time by extracting up to 20 μg high-purity plasmid or cosmid in 30 minutes or less. Plasmid DNA purified with MQ Plasmid Extraction Kits is immediately ready for use. The extracted plasmid DNA is suitable for a variety of experiments in molecular biology, including automated fluorescent DNA sequencing, restriction enzyme digestion, or transformation.
1. MQ Plasmid Extraction Kit procedure is simple, fast, and efficient.
2. It prepares high quality Plasmid DNA that can be used in any downstream applications such as sequencing, PCR, cloning, transformation, or restriction digestions.
3. The protocol is reproducible.
The MQ Plasmid Extraction Kit provides a simple and efficient method for mini plasmid DNA purification. The plasmid DNA is selectively adsorbed in silica gel-based MQ column and other impurities such as proteins, salts, nucleotides, oligos (<40-mer) are washed away. In order to maximize the recovery yield of plasmid DNA, a color indicator—VisualLyse—is added to the buffer which prevents insufficient or over-lysis during the lysis and neutralization step. The plasmid DNA is then eluted off the column and can be used for any downstream application.
MQ Plasmid DNA Extraction Kit
MQ Plasmid DNA Extraction Kit uses alkaline lysis method to lyse cells, and specifically extract plasmid DNA based on the principle that the centrifugal adsorption column specifically binds to the DNA in the solution under high salt conditions. The silicon matrix material used in the centrifugal adsorption column can adsorb DNA efficiently and specifically, and can remove impurity proteins and other organic compounds in cells to the greatest extent. The plasmid DNA extracted by this kit can be applied to various routine operations, including enzyme digestion, PCR, sequencing, ligation, and transformation tests.
1. MQ Plasmid Extraction Kit procedure is simple, fast, and efficient.
2. It prepares high quality Plasmid DNA that can be used in any downstream applications such as sequencing, PCR, cloning, transformation, or restriction digestions.
3. The protocol is reproducible.
The MQ Plasmid Extraction Kit provides a simple and efficient method for mini plasmid DNA purification. The plasmid DNA is selectively adsorbed in silica gel-based MQ column and other impurities such as proteins, salts, nucleotides, oligos (<40-mer) are washed away. The plasmid DNA is then eluted off the column and can be used for any downstream application.
MQ Plasmid DNA Maxi Extraction Kit
MQ Plasmid DNA Maxi Extraction Kit is an excellent tool offering a rapid and economic method to purify plasmid DNA from bacterial cultures. This technology is based on alkaline lysis and purification by anion-exchange chromatography. Compared with other harmful and time-consuming procedures such as phenol-chloroform extraction and ethanol precipitation, MQ Plasmid DNA Maxi Extraction Kit shortens the handling time to about 2 hours. The high quality plasmid DNA can be used directly for any downstream application.
1. MQ Plasmid DNA Maxi Extraction Kit procedure is simple, fast, and efficient.
2. It prepares high quality Plasmid DNA that can be used in any downstream applications such as sequencing, PCR, cloning, transformation, or restriction digestions.
3. The protocol is reproducible.
4. For 100-250 ml of bacterial culture for high copy plasmids and 200-400 ml of bacterial culture for low copy plasmids, this kit yields up to 500 µg for high copy plasmids in about 2 hours.
The MQ Plasmid DNA Maxi Extraction Kit provides a simple and efficient method for mini plasmid DNA purification. The plasmid DNA is selectively adsorbed in silica gel-based MQ column and other impurities such as proteins, salts, nucleotides, oligos (<40-mer) are washed away.
MQ Viral DNA Extraction Kit
MQ Viral DNA Extraction Kit provides a fast, simple and highly reproducible method for isolation of viral DNA from broad range cell-free clinical samples including serum, urine and plasma for clinical research and life science applications. Viral DNA in lysates is selectively absorbed in spin column and other impurities don't bind in the column. The procedure is simple and fast, no phenol extraction is required. Purified viral DNA can be used for PCR, Real Time PCR and other clinical research applications.
1. Fast and easy processing using a rapid spin-column format. The entire procedure takes approx. 20 minutes only.
2. Sensitive. 30-50 virus particles in 1 ml of sample can be detected by PCR.
3. No phenol/chloroform and no ethanol precipitation are required.
4. Compatible with PCR, Real Time PCR and other clinical applications.
5. Suitable for broad range cell-free clinical samples including serum, urine and plasma.
6. No toxic. The kit does not contain toxic reagents.
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