MOLEQULE-ON reagents and kits are essential tool in research and various field of science, which enable accurate experiments and tests. Our products are integrated with newest technologies to meet the demand of a wide range of laboratories. The list includes PCR and qPCR reagents, electrophoresis, enzymes, nucleic acid extraction and purification kits, colorimetric and ELISA kits and buffers.
Phosphate Buffered Saline (1X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 1X Working Solution. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Phosphate Buffered Saline (10X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 10X concentrate. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
To make one liter of 1X working concentration of PBS, mix 100ml of 10X Phosphate B uffered Saline in 900ml of sterile distilled water. In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Phosphate Buffered Saline (1X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 1X Working Solution. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Phosphate Buffered Saline (10X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 10X concentrate. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
To make one liter of 1X working concentration of PBS, mix 100ml of 10X Phosphate B uffered Saline in 900ml of sterile distilled water. In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Tris Borate EDTA Buffer, TBE, 1X
TBE buffer (Tris-Borate-EDTA), 1X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Borate EDTA Buffer, TBE, 5X
TBE buffer (Tris-Borate-EDTA), 5X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
5X solution of TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Borate EDTA, TBE Buffer, 10X
TBE buffer (Tris-Borate-EDTA), 10X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
10X solution of TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Borate EDTA Buffer, TBE, 1X
TBE buffer (Tris-Borate-EDTA), 1X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Borate EDTA Buffer, TBE, 5X
TBE buffer (Tris-Borate-EDTA), 5X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
5X solution of TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Borate EDTA, TBE Buffer, 10X
TBE buffer (Tris-Borate-EDTA), 10X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
10X solution of TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Acetate EDTA Buffer, TAE, 10X
TAE (Tris-Acetate-EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double-stranded DNA runs faster with TAE buffer. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA and can also be used as both a running buffer and a gel preparation buffer. It is recommended for the resolution of RNA and DNA fragments larger than 1500 base pairs, for genomic DNA, and for large supercoiled DNA.
MOLEQULE-ON TAE Buffer is free from DNase, RNase, and Protease activity.
Tris Acetate EDTA Buffer, TAE, 25X
TAE (Tris-Acetate-EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double-stranded DNA runs faster with TAE buffer. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and also as both a running buffer and a gel preparation buffer. It is recommended for the resolution of RNA and DNA fragments larger than 1500 base pairs, for genomic DNA, and for large supercoiled DNA.
MOLEQULE-ON TAE Buffer is free from DNase, RNase, and Protease activity.
Tris Acetate EDTA Buffer, TAE, 10X
TAE (Tris-Acetate-EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double-stranded DNA runs faster with TAE buffer. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA and can also be used as both a running buffer and a gel preparation buffer. It is recommended for the resolution of RNA and DNA fragments larger than 1500 base pairs, for genomic DNA, and for large supercoiled DNA.
MOLEQULE-ON TAE Buffer is free from DNase, RNase, and Protease activity.
Tris Acetate EDTA Buffer, TAE, 25X
TAE (Tris-Acetate-EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double-stranded DNA runs faster with TAE buffer. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and also as both a running buffer and a gel preparation buffer. It is recommended for the resolution of RNA and DNA fragments larger than 1500 base pairs, for genomic DNA, and for large supercoiled DNA.
MOLEQULE-ON TAE Buffer is free from DNase, RNase, and Protease activity.
Tris-EDTA (TE) Buffer 1X
TE buffer is a mixture of Tris and EDTA, commonly used as a dissolver or preservative. Tris maintains a stable pH in biological samples and provides buffering capacity, while EDTA is a metal chelator that prevents metal ion interference by chelating divalent metal ions. TE Buffer is frequently used in molecular biology techniques, such as DNA and RNA sample preparation, enzymatic reactions, and electrophoresis. It maintains the stability and integrity of DNA and RNA by inhibiting the activity of metal-dependent enzymes and nucleases.
TE buffer is useful for general extraction, washing, suspension, and storage buffer, as well as in the preparation of nucleic acids. It is also commonly used for PCR, DNA sequencing, restriction enzyme digestion, and ligation.
Tris-EDTA (TE) Buffer 10X
TE buffer is a mixture of Tris and EDTA, generally used as a dissolver or preservative. Tris maintains a stable pH in biological samples and provides buffering capacity, while EDTA is a metal chelator that helps prevent metal ion interference by chelating divalent metal ions. TE buffer is frequently used in molecular biology techniques, such as DNA and RNA sample preparation, enzymatic reactions, and electrophoresis. It maintains the stability and integrity of DNA and RNA by inhibiting the activity of metal-dependent enzymes and nucleases.
TE buffer is useful for general extraction, washing, suspension, and storage buffer, as well as in the preparation of nucleic acids. It is also commonly used for PCR, DNA sequencing, restriction enzyme digestion, and ligation.
Tris-EDTA (TE) Buffer 1X
TE buffer is a mixture of Tris and EDTA, commonly used as a dissolver or preservative. Tris maintains a stable pH in biological samples and provides buffering capacity, while EDTA is a metal chelator that prevents metal ion interference by chelating divalent metal ions. TE Buffer is frequently used in molecular biology techniques, such as DNA and RNA sample preparation, enzymatic reactions, and electrophoresis. It maintains the stability and integrity of DNA and RNA by inhibiting the activity of metal-dependent enzymes and nucleases.
TE buffer is useful for general extraction, washing, suspension, and storage buffer, as well as in the preparation of nucleic acids. It is also commonly used for PCR, DNA sequencing, restriction enzyme digestion, and ligation.
Tris-EDTA (TE) Buffer 10X
TE buffer is a mixture of Tris and EDTA, generally used as a dissolver or preservative. Tris maintains a stable pH in biological samples and provides buffering capacity, while EDTA is a metal chelator that helps prevent metal ion interference by chelating divalent metal ions. TE buffer is frequently used in molecular biology techniques, such as DNA and RNA sample preparation, enzymatic reactions, and electrophoresis. It maintains the stability and integrity of DNA and RNA by inhibiting the activity of metal-dependent enzymes and nucleases.
TE buffer is useful for general extraction, washing, suspension, and storage buffer, as well as in the preparation of nucleic acids. It is also commonly used for PCR, DNA sequencing, restriction enzyme digestion, and ligation.
Tris Glycine Buffer, 10X
Tris-Glycine-SDS (TGS) running buffer is the most commonly used buffer for native protein gel electrophoresis or transfer buffer for western blots. TGS buffer is usually used for both anode and cathode buffer. 10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.3) running buffer. It can be used for semi-dry blotting for SDS-PAGE gels by the addition of 20% methanol.
Recommended running conditions are 150 volts for mini vertical gel electrophoresis units.
Tris Glycine SDS Buffer, 10X
Tris-Glycine-SDS (TGS) running buffer is the most commonly used buffer for SDS-PAGE of proteins. TGS is usually used for both anode and cathode buffer. 10X Tris-Glycine-SDS Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine-SDS (pH 8.3) running buffer used for SDS-PAGE (protein polyacrylamide gel electrophoresis). Recommended running conditions are 150 volts for mini vertical gel electrophoresis units.
Tris Buffered Saline Buffer, TBS, 20X
Tris Buffered Saline (TBS) is a buffering solution that stabilizes pH. The solution is commonly used for western blot and ELISA procedures. It is used as a convenient prepared buffer for use in various applications.
To make one liter of 1X working concentration, take 50 ml of 20X TBS Buffer and mix with 950 ml of sterile distilled water. 1X Buffer contains 0.14 M Sodium Chloride, 3 mM Potassium Chloride, and 25 mM Tris Base.
Tris Buffer, 1M
Tris (hydroxymethyl)-aminomethane hydrochloride buffer in 1M concentration is the most suitable solution to be used in a variety of biological applications. The buffer is mostly used to control pH in different molecular biology procedures.
Phosphate Buffer, 1M
Phosphate buffers (also called Gomori buffers) consist of potassium phosphate monobasic and potassium phosphate dibasic salts. The buffer is soluble in water and has high buffering capacity. Phosphate buffer is used in different research domains, including molecular biology.
Carbonate buffer
Carbonate buffers contain salt of sodium carbonate and sodium bicarbonate. Carbonate buffer is important for numerous diverse cellular processes. It is important in the acid-base homeostasis and widely used as buffering system to maintain the pH of blood.
Sodium Chloride Solution, 1M
Sodium chloride 1M solution is widely used in different procedures of scientific domains like biology and chemistry. 1M solution of NaCl provides a stable ionic strength which is required for many biochemical and biophysical processes. During experimentation, 1M solution isotonic solution of sodium chloride salt helps in maintaining cellular integrity. Isotonic solution of NaCl prevents cells from shrinking or lysis when they are under study. It is also used in nucleic acid extractions for enhancing the capability of nucleic acid to precipitate by disturbing their hydrogen bonds in the presence of alcohol.
RIPA Buffer
RIPA buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane, and nuclear proteins and is compatible with many applications, including reporter assays, protein assays, immunoassays, and protein purification.
Extraction of cellular proteins requires efficient cell lysis and protein solubilization, while avoiding protein degradation and/or interference with protein immunoreactivity and biological activity. RIPA Buffer enables rapid, efficient cell lysis and solubilization of protein from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and wash buffer for small-scale affinity pull-down applications, such as immunoprecipitation, since most antibodies and protein antigens are not adversely affected by the components of this buffer. In addition, RIPA buffer minimizes non-specific protein-binding most specific interactions to occur, enabling studies of relevant protein-protein interactions.
RIPA buffer is supplied as several kinds of solution. Protease and phosphatase inhibitors may be added to the lysis buffer as needed. One ml of the RIPA buffer is sufficient to lyse cells from one 100mm culture dish (0.5 to 5X10^7 cells) of most adherent mammalian cell lines.
1. Remove growth medium from the cells by decantation or aspiration.
2. Wash cells to remove residual medium. Slowly add a volume of a physiological wash solution, such as DPBS, equal to the original medium volume being careful not to dislodge cells. Mix gently and remove the wash solution. Repeat the wash once in order to remove any other minor contaminants. More washing steps can be done, but two is usually sufficient to remove nearly all of the contaminants.
3. After removal of the final wash solution from the cells, add an appropriate volume of RIPA Buffer (1ml for 0.5 to 5x10^7 cells). Incubate on ice or in a refrigerator (2-8°C) for five minutes.
4. Rapidly scrape the plate with a cell scraper to remove and lyse residual cells. Transfer the cell lysate to a tube on ice. The lysate can either be used immediately or quick-frozen in liquid nitrogen and stored at -70°C for future use. It is best to freeze the lysate before clarification, since the freeze-thaw cycle may cause some denatured protein aggregates.
5. Clarify the lysate by centrifugation at 8,000 x g for 10 minutes at 4°C to pellet the cell debris.
Note: if a mucoid aggregate of denatured nucleic acids is present, carefully remove it with a micropipette before centrifugation.
6. Carefully transfer the supernatant containing the soluble protein to a tube on ice for immunoprecipitation or other analysis.
1. Pellet the cells by centrifugation at 450 x g for 5 minutes.
2. Carefully remove the medium from the cell pellet by decantation or aspiration.
3. Wash the cells to remove residual medium. Add a volume of a physiological wash solution, such as DPBS, equal to the original medium volume. Mix or vortex briefly to resuspend the cells completely. Centrifuge for 5 minutes at 450 x g to pellet the cells and carefully remove wash solution supernatant. Repeat the wash once in order to remove any other minor contaminants. More washing steps can be done, but two is usually sufficient to remove nearly all of the contaminants.
4. After removal of the final wash solution from the cells, add an appropriate volume of RIPA Buffer (1ml per 0.5 to 5X10^7 cells) to the cell pellet, and mix or vortex briefly to resuspend the cells completely. Incubate on ice or in a refrigerator (2-8°C) for five minutes. Vortex briefly to resuspend and lyse residual cells.
5. The lysate can either be used immediately or quick-frozen in liquid nitrogen and stored at -70°C for future use.
6. Clarify the lysate by centrifugation at 8,000 x g for 10 minutes at 4°C to pellet the cell debris.
Note: If a mucoid aggregate of denatured nucleic acids is present, carefully remove it with a micropipette before centrifugation.
7. Carefully transfer the supernatant containing the soluble protein to a tube on ice for immunoprecipitation or other analysis.
Red Cell Lysis Buffer
MQ RBC Lysis Buffer uses the principle that the salt ion concentration inside and outside the cell causes the cell membrane to burst to split non-nucleated red blood cells.
This lysis buffer has been treated aseptically and is mainly used for the separation and purification of histiocytes digested and dispersed by enzymes, the separation and purification of lymphocytes, and the removal of red blood cells in experiments such as the extraction of histiocytic protein and nucleic acid.
MQ Western One Step Blocker
MQ Western One Step Blocker is a blocking solution for Western blot analysis. This Blocker buffer not only provides blocking and primary and secondary antibody hybridization in one step but also enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. It, therefore, serves as both blocker and enhancer in Western analysis. With the three - in - one step procedure, MQ Western One Step Blocker is a time and labor economic solution for the time consuming and laborious Western procedure.
1°Ab
(ab
8227,
abcam
):
Rabbit
anti
beta
actin;
1:5,000
2°Ab
(ab
205718,
abcam
):
Goat
anti
Rabbit
HRP;
1:5,000
MQ Western Stripping Buffer
Nitrocellulo se and PVDF membranes probed by Western blotting procedures and detected by chemiluminescent or other non - precipitating substrates can be stripped and re - probed using MQ Western Stripping Buffer. Western blotting is widely used to detect and compare proteins in complex mixtures, and chemiluminescence has largely replaced chromogenic substrates as the most convenient and sensitive method of detection. One advantage of chemiluminescence is the ability to strip and re - probe the protein mixture on the membrane. Traditional stripping methods use conditions that are effective for only low - affinity antibody - antigen interactions or are so harsh that they tend to adversely alter the antigen for subsequent immunoprobing. MQ Western Stripping Buffer is a robust but gentle formulation for stripping primary and secondary antibodies from blots to enable several reprobings on the same membrane.
HEPES Buffer, 1M
HEPES (N'-hydroxythylpiperazine-N'-ethanesulfanic acid) is a hydrogen ion buffer that can control a constant pH range for a long time. The effective buffer range is pH 6.8-8.2. It is often used to prepare protein extraction buffer and cell culture buffer.
HEPES is used in cell culture with a final concentration of 10-25 mM, which can maintain a relatively constant pH value in open culture or cell observation.
PIPES Buffer, 1M
PIPES (Piperazine-N,N'-bis(2-ethanesulfonic acid)) is frequently used as a buffer solution in biochemistry. PIPES reduces lipid loss when buffering glutaraldehyde histology in plant and animal tissues. It also has an insignificant capacity to bind divalent ions. PIPES is suitable for cell culture work as the pKa is close to the physiological pH.
TNE Buffer
TNE Buffer contains Tris Base (10mM), NaCl (100mM) and EDTA (1mM) pH 7.4. TNE Buffer is often used for the fluorometric determination of DNA content, resuspension of pelleted virus or reporter virus particles and isolation of proteins from yeast cells.
Triton X100 Detergent Solution
Triton X-100 is a commonly used detergent in various laboratories. Triton X-100 Detergent Solution contains 10% highly purified Triton X-100 which is used as a non-ionic detergent for the isolation of membrane proteins under non-denaturing conditions. It is used as a multi-purpose detergent to optimize protein-protein interactions.
Phosphate Buffered Saline (1X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 1X Working Solution. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Phosphate Buffered Saline (10X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 10X concentrate. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
To make one liter of 1X working concentration of PBS, mix 100ml of 10X Phosphate B uffered Saline in 900ml of sterile distilled water. In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Phosphate Buffered Saline (1X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 1X Working Solution. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Phosphate Buffered Saline (10X)
Phosphate buffered saline (PBS) from MOLEQULE - ON available as a 10X concentrate. It is filtered through 0.2 μm filter membrane. The buffer is isotonic and non - toxic to most cells therefore, it is used in many biological research procedures. PBS Buffer commonly used as substance dilution, cell wash solution and rinsing of cell container.
To make one liter of 1X working concentration of PBS, mix 100ml of 10X Phosphate B uffered Saline in 900ml of sterile distilled water. In 1X solution, the concentration of phosphate buffer is 0.01M while of sodium chloride is 0.154M. The solution pH will be ~7.2 - 7.4.
Tris Borate EDTA Buffer, TBE, 1X
TBE buffer (Tris-Borate-EDTA), 1X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Borate EDTA Buffer, TBE, 5X
TBE buffer (Tris-Borate-EDTA), 5X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
5X solution of TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Borate EDTA, TBE Buffer, 10X
TBE buffer (Tris-Borate-EDTA), 10X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
10X solution of TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Borate EDTA Buffer, TBE, 1X
TBE buffer (Tris-Borate-EDTA), 1X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Borate EDTA Buffer, TBE, 5X
TBE buffer (Tris-Borate-EDTA), 5X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
5X solution of TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Borate EDTA, TBE Buffer, 10X
TBE buffer (Tris-Borate-EDTA), 10X solution, is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments, whereas TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. 1X TBE Solution contains 0.089 M Tris Base, 0.089 M Borate Acid, and 0.002 M EDTA.
10X solution of TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Please note if crystallization or precipitation does occur, additional filtration may be necessary. The solution should be filtered through a 0.2 μm cellulose acetate or cellulose nitrate filter. MOLEQULE-ON TBE Buffer is free from DNase, RNase, and Protease activity.
Tris Acetate EDTA Buffer, TAE, 10X
TAE (Tris-Acetate-EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double-stranded DNA runs faster with TAE buffer. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA and can also be used as both a running buffer and a gel preparation buffer. It is recommended for the resolution of RNA and DNA fragments larger than 1500 base pairs, for genomic DNA, and for large supercoiled DNA.
MOLEQULE-ON TAE Buffer is free from DNase, RNase, and Protease activity.
Tris Acetate EDTA Buffer, TAE, 25X
TAE (Tris-Acetate-EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double-stranded DNA runs faster with TAE buffer. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and also as both a running buffer and a gel preparation buffer. It is recommended for the resolution of RNA and DNA fragments larger than 1500 base pairs, for genomic DNA, and for large supercoiled DNA.
MOLEQULE-ON TAE Buffer is free from DNase, RNase, and Protease activity.
Tris Acetate EDTA Buffer, TAE, 10X
TAE (Tris-Acetate-EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double-stranded DNA runs faster with TAE buffer. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA and can also be used as both a running buffer and a gel preparation buffer. It is recommended for the resolution of RNA and DNA fragments larger than 1500 base pairs, for genomic DNA, and for large supercoiled DNA.
MOLEQULE-ON TAE Buffer is free from DNase, RNase, and Protease activity.
Tris Acetate EDTA Buffer, TAE, 25X
TAE (Tris-Acetate-EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double-stranded DNA runs faster with TAE buffer. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and also as both a running buffer and a gel preparation buffer. It is recommended for the resolution of RNA and DNA fragments larger than 1500 base pairs, for genomic DNA, and for large supercoiled DNA.
MOLEQULE-ON TAE Buffer is free from DNase, RNase, and Protease activity.
Tris-EDTA (TE) Buffer 1X
TE buffer is a mixture of Tris and EDTA, commonly used as a dissolver or preservative. Tris maintains a stable pH in biological samples and provides buffering capacity, while EDTA is a metal chelator that prevents metal ion interference by chelating divalent metal ions. TE Buffer is frequently used in molecular biology techniques, such as DNA and RNA sample preparation, enzymatic reactions, and electrophoresis. It maintains the stability and integrity of DNA and RNA by inhibiting the activity of metal-dependent enzymes and nucleases.
TE buffer is useful for general extraction, washing, suspension, and storage buffer, as well as in the preparation of nucleic acids. It is also commonly used for PCR, DNA sequencing, restriction enzyme digestion, and ligation.
Tris-EDTA (TE) Buffer 10X
TE buffer is a mixture of Tris and EDTA, generally used as a dissolver or preservative. Tris maintains a stable pH in biological samples and provides buffering capacity, while EDTA is a metal chelator that helps prevent metal ion interference by chelating divalent metal ions. TE buffer is frequently used in molecular biology techniques, such as DNA and RNA sample preparation, enzymatic reactions, and electrophoresis. It maintains the stability and integrity of DNA and RNA by inhibiting the activity of metal-dependent enzymes and nucleases.
TE buffer is useful for general extraction, washing, suspension, and storage buffer, as well as in the preparation of nucleic acids. It is also commonly used for PCR, DNA sequencing, restriction enzyme digestion, and ligation.
Tris-EDTA (TE) Buffer 1X
TE buffer is a mixture of Tris and EDTA, commonly used as a dissolver or preservative. Tris maintains a stable pH in biological samples and provides buffering capacity, while EDTA is a metal chelator that prevents metal ion interference by chelating divalent metal ions. TE Buffer is frequently used in molecular biology techniques, such as DNA and RNA sample preparation, enzymatic reactions, and electrophoresis. It maintains the stability and integrity of DNA and RNA by inhibiting the activity of metal-dependent enzymes and nucleases.
TE buffer is useful for general extraction, washing, suspension, and storage buffer, as well as in the preparation of nucleic acids. It is also commonly used for PCR, DNA sequencing, restriction enzyme digestion, and ligation.
Tris-EDTA (TE) Buffer 10X
TE buffer is a mixture of Tris and EDTA, generally used as a dissolver or preservative. Tris maintains a stable pH in biological samples and provides buffering capacity, while EDTA is a metal chelator that helps prevent metal ion interference by chelating divalent metal ions. TE buffer is frequently used in molecular biology techniques, such as DNA and RNA sample preparation, enzymatic reactions, and electrophoresis. It maintains the stability and integrity of DNA and RNA by inhibiting the activity of metal-dependent enzymes and nucleases.
TE buffer is useful for general extraction, washing, suspension, and storage buffer, as well as in the preparation of nucleic acids. It is also commonly used for PCR, DNA sequencing, restriction enzyme digestion, and ligation.
Tris Glycine Buffer, 10X
Tris-Glycine-SDS (TGS) running buffer is the most commonly used buffer for native protein gel electrophoresis or transfer buffer for western blots. TGS buffer is usually used for both anode and cathode buffer. 10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.3) running buffer. It can be used for semi-dry blotting for SDS-PAGE gels by the addition of 20% methanol.
Recommended running conditions are 150 volts for mini vertical gel electrophoresis units.
Tris Glycine SDS Buffer, 10X
Tris-Glycine-SDS (TGS) running buffer is the most commonly used buffer for SDS-PAGE of proteins. TGS is usually used for both anode and cathode buffer. 10X Tris-Glycine-SDS Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine-SDS (pH 8.3) running buffer used for SDS-PAGE (protein polyacrylamide gel electrophoresis). Recommended running conditions are 150 volts for mini vertical gel electrophoresis units.
Buffer, TBS, 20X
Tris Buffered Saline Buffer, TBS, 20X
Tris Buffered Saline (TBS) is a buffering solution that stabilizes pH. The solution is commonly used for western blot and ELISA procedures. It is used as a convenient prepared buffer for use in various applications.
To make one liter of 1X working concentration, take 50 ml of 20X TBS Buffer and mix with 950 ml of sterile distilled water. 1X Buffer contains 0.14 M Sodium Chloride, 3 mM Potassium Chloride, and 25 mM Tris Base.
Tris Buffer, 1M
Tris (hydroxymethyl)-aminomethane hydrochloride buffer in 1M concentration is the most suitable solution to be used in a variety of biological applications. The buffer is mostly used to control pH in different molecular biology procedures.
Phosphate Buffer, 1M
Phosphate buffers (also called Gomori buffers) consist of potassium phosphate monobasic and potassium phosphate dibasic salts. The buffer is soluble in water and has high buffering capacity. Phosphate buffer is used in different research domains, including molecular biology.
Carbonate buffer
Carbonate buffers contain salt of sodium carbonate and sodium bicarbonate. Carbonate buffer is important for numerous diverse cellular processes. It is important in the acid-base homeostasis and widely used as buffering system to maintain the pH of blood.
Sodium Chloride Solution, 1M
Sodium chloride 1M solution is widely used in different procedures of scientific domains like biology and chemistry. 1M solution of NaCl provides a stable ionic strength which is required for many biochemical and biophysical processes. During experimentation, 1M solution isotonic solution of sodium chloride salt helps in maintaining cellular integrity. Isotonic solution of NaCl prevents cells from shrinking or lysis when they are under study. It is also used in nucleic acid extractions for enhancing the capability of nucleic acid to precipitate by disturbing their hydrogen bonds in the presence of alcohol.
RIPA Buffer
RIPA buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane, and nuclear proteins and is compatible with many applications, including reporter assays, protein assays, immunoassays, and protein purification.
Extraction of cellular proteins requires efficient cell lysis and protein solubilization, while avoiding protein degradation and/or interference with protein immunoreactivity and biological activity. RIPA Buffer enables rapid, efficient cell lysis and solubilization of protein from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and wash buffer for small-scale affinity pull-down applications, such as immunoprecipitation, since most antibodies and protein antigens are not adversely affected by the components of this buffer. In addition, RIPA buffer minimizes non-specific protein-binding most specific interactions to occur, enabling studies of relevant protein-protein interactions.
RIPA buffer is supplied as several kinds of solution. Protease and phosphatase inhibitors may be added to the lysis buffer as needed. One ml of the RIPA buffer is sufficient to lyse cells from one 100mm culture dish (0.5 to 5X10^7 cells) of most adherent mammalian cell lines.
1. Remove growth medium from the cells by decantation or aspiration.
2. Wash cells to remove residual medium. Slowly add a volume of a physiological wash solution, such as DPBS, equal to the original medium volume being careful not to dislodge cells. Mix gently and remove the wash solution. Repeat the wash once in order to remove any other minor contaminants. More washing steps can be done, but two is usually sufficient to remove nearly all of the contaminants.
3. After removal of the final wash solution from the cells, add an appropriate volume of RIPA Buffer (1ml for 0.5 to 5x10^7 cells). Incubate on ice or in a refrigerator (2-8°C) for five minutes.
4. Rapidly scrape the plate with a cell scraper to remove and lyse residual cells. Transfer the cell lysate to a tube on ice. The lysate can either be used immediately or quick-frozen in liquid nitrogen and stored at -70°C for future use. It is best to freeze the lysate before clarification, since the freeze-thaw cycle may cause some denatured protein aggregates.
5. Clarify the lysate by centrifugation at 8,000 x g for 10 minutes at 4°C to pellet the cell debris.
Note: if a mucoid aggregate of denatured nucleic acids is present, carefully remove it with a micropipette before centrifugation.
6. Carefully transfer the supernatant containing the soluble protein to a tube on ice for immunoprecipitation or other analysis.
1. Pellet the cells by centrifugation at 450 x g for 5 minutes.
2. Carefully remove the medium from the cell pellet by decantation or aspiration.
3. Wash the cells to remove residual medium. Add a volume of a physiological wash solution, such as DPBS, equal to the original medium volume. Mix or vortex briefly to resuspend the cells completely. Centrifuge for 5 minutes at 450 x g to pellet the cells and carefully remove wash solution supernatant. Repeat the wash once in order to remove any other minor contaminants. More washing steps can be done, but two is usually sufficient to remove nearly all of the contaminants.
4. After removal of the final wash solution from the cells, add an appropriate volume of RIPA Buffer (1ml per 0.5 to 5X10^7 cells) to the cell pellet, and mix or vortex briefly to resuspend the cells completely. Incubate on ice or in a refrigerator (2-8°C) for five minutes. Vortex briefly to resuspend and lyse residual cells.
5. The lysate can either be used immediately or quick-frozen in liquid nitrogen and stored at -70°C for future use.
6. Clarify the lysate by centrifugation at 8,000 x g for 10 minutes at 4°C to pellet the cell debris.
Note: If a mucoid aggregate of denatured nucleic acids is present, carefully remove it with a micropipette before centrifugation.
7. Carefully transfer the supernatant containing the soluble protein to a tube on ice for immunoprecipitation or other analysis.
Red Cell Lysis Buffer
MQ RBC Lysis Buffer uses the principle that the salt ion concentration inside and outside the cell causes the cell membrane to burst to split non-nucleated red blood cells.
This lysis buffer has been treated aseptically and is mainly used for the separation and purification of histiocytes digested and dispersed by enzymes, the separation and purification of lymphocytes, and the removal of red blood cells in experiments such as the extraction of histiocytic protein and nucleic acid.
MQ Western One Step Blocker
MQ Western One Step Blocker is a blocking solution for Western blot analysis. This Blocker buffer not only provides blocking and primary and secondary antibody hybridization in one step but also enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. It, therefore, serves as both blocker and enhancer in Western analysis. With the three - in - one step procedure, MQ Western One Step Blocker is a time and labor economic solution for the time consuming and laborious Western procedure.
1°Ab
(ab
8227,
abcam
):
Rabbit
anti
beta
actin;
1:5,000
2°Ab
(ab
205718,
abcam
):
Goat
anti
Rabbit
HRP;
1:5,000
MQ Western Stripping Buffer
Nitrocellulo se and PVDF membranes probed by Western blotting procedures and detected by chemiluminescent or other non - precipitating substrates can be stripped and re - probed using MQ Western Stripping Buffer. Western blotting is widely used to detect and compare proteins in complex mixtures, and chemiluminescence has largely replaced chromogenic substrates as the most convenient and sensitive method of detection. One advantage of chemiluminescence is the ability to strip and re - probe the protein mixture on the membrane. Traditional stripping methods use conditions that are effective for only low - affinity antibody - antigen interactions or are so harsh that they tend to adversely alter the antigen for subsequent immunoprobing. MQ Western Stripping Buffer is a robust but gentle formulation for stripping primary and secondary antibodies from blots to enable several reprobings on the same membrane.
HEPES Buffer, 1M
HEPES (N'-hydroxythylpiperazine-N'-ethanesulfanic acid) is a hydrogen ion buffer that can control a constant pH range for a long time. The effective buffer range is pH 6.8-8.2. It is often used to prepare protein extraction buffer and cell culture buffer.
HEPES is used in cell culture with a final concentration of 10-25 mM, which can maintain a relatively constant pH value in open culture or cell observation.
PIPES Buffer, 1M
PIPES (Piperazine-N,N'-bis(2-ethanesulfonic acid)) is frequently used as a buffer solution in biochemistry. PIPES reduces lipid loss when buffering glutaraldehyde histology in plant and animal tissues. It also has an insignificant capacity to bind divalent ions. PIPES is suitable for cell culture work as the pKa is close to the physiological pH.
TNE Buffer
TNE Buffer contains Tris Base (10mM), NaCl (100mM) and EDTA (1mM) pH 7.4. TNE Buffer is often used for the fluorometric determination of DNA content, resuspension of pelleted virus or reporter virus particles and isolation of proteins from yeast cells.
Triton X100 Detergent Solution
Triton X-100 is a commonly used detergent in various laboratories. Triton X-100 Detergent Solution contains 10% highly purified Triton X-100 which is used as a non-ionic detergent for the isolation of membrane proteins under non-denaturing conditions. It is used as a multi-purpose detergent to optimize protein-protein interactions.
