Enzymes
MOLEQULE-ON reagents and kits are essential tool in research and various field of science, which enable accurate experiments and tests. Our products are integrated with newest technologies to meet the demand of a wide range of laboratories. The list includes PCR and qPCR reagents, electrophoresis, enzymes, nucleic acid extraction and purification kits, colorimetric and ELISA kits and buffers.
Proteinase K
Proteinase K is a broad spectrum serine protease useful for general digestion of proteins. MOLEQULE – ON Proteinase K is highly active and stable in SDS, EDTA, urea and at high temperatures. Its activity is greater than 30 units/mg at 37°C
Applications
MOLEQULE-ON Proteinase K can be used in the preparation of RNA and high molecular weight DNA. It can be used in the preparation of tissue sections for in situ hybridization. It can also be used in the inactivation of nucleases during nucleic acid extraction and lysis of cells to facilitate the isolation of intact genomic DNA or RNA.
Product Name
Proteinase K
Catalog Number
EN-M-001-100
RNAse A
Ribonuclease A (RNase A) is an endoribonuclease that attacks at the 3’OH phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved into pG-pG-pCp and A-pG. The highest activity is exhibited with single-stranded RNA.
Applications
RNase A is used to remove RNA from genomic DNA and plasmid preparations. It is also used in RNA sequence analysis and protection assays.
Product Name
RNAse A
Catalog Number
EN-M-002-100
DNase I
MOLEQULE-ON DNase I is an RNase-free endonuclease that cleaves the phosphodiester bonds in single and double-stranded DNA. The activity of the enzyme depends on the presence of Ca²⁺, Mg²⁺, or Mn²⁺ ions. It is used in DNA-free RNA preparation, which is used for RT-PCR and real-time PCR. It can also be used in the removal of DNA templates for in vitro transcription.
Product Name
DNase I
Catalog Number
EN-M-003-100
Diastase
MOLEQULE-ON Diastase is α-Amylase ex. Malt (Fungal Diastase ex. Aspergillus oryzae) 1:2000 (2000 U/g powder). Fungal Diastase or Fungal Alpha-Amylase enhances the conversion of starch into its by-products, i.e., maltose, and maltose to glucose. It is a starch-hydrolyzing enzyme that breaks down complex carbohydrates (polysaccharides, i.e., starch) into simple carbohydrates (monosaccharides, i.e., glucose).
Product Name
Diastase
Catalog Number
EN-M-004-100
MQ Reverse Transcriptase
MQ Rscript Reverse Transcriptase is engineered innovatively and specifically for Research and Laboratory applications for meeting all your cDNA synthesis needs and for overcoming the most challenging secondary RNA structures over a wide temperature range. The Kit contains our next-generation, engineered recombinant M-MLV reverse transcriptase, with improved thermostability, processivity, robustness, optimal cDNA yields, proprietary site mutations for reduced RNase H activity, and extended half-life, is the most versatile reverse transcriptase in the world for not only simply meeting the routine cDNA synthesis requirements but also enabling superior performance for even the most challenging RNA samples at hand.
Stability of MQ RScript Reverse Transcriptase
The stability RT – qPCR data shows the performance of MQ RScript reverse transcriptase is maintained without any significant alteration in performance at 37 ° C after a period of 3 weeks ( 21 days), demonstrating its high resistance to temperature and time . The percentage of MQ RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature.
Performance of One – Step RT – qPCR with MQ RScript Reverse Transcriptase
The stability RT – qPCR data shows the performance of MQ RScript reverse transcriptase is maintained without any significant alteration in performance at 37 ° C after a period of 3 weeks ( 21 days), demonstrating its high resistance to temperature and time . The percentage of MQ RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature.
Enhancement of the Thermostability
Reverse transcription of a 1 kb fragment ARHGAP 29 RNA using MQ RScript reverse transcriptase or competitor reverse transcriptases were used to carry this experiment . Regardless of the variations in temperatures ( 42 – 60 ° C) MQ RScript reverse transcriptase shows a stable and superior performance at higher temperatures of up to 60 °C compared to other brands . The molecular weight marker used was DNA Ladder 1 kb.
Elongation of R N A templates up to 11 kb in length
MQ RScript reverse transcriptase was used in a reaction with a range of human bladder cancer cell lines – 5637 (HTB – 9) RNA. The resulting synthesized cDNA (8 kb ,11kb) was followed by PCR and visualized on a 2% agarose gel. The molecular weight marker used was DNA Ladder 1kb
At a temperature of 55 ° C, MQ RScript Reverse Transcriptase exceeds performance demonstrating better efficiency and higher cDNA yields . MQ RScript Reverse Transcriptase shows a stable and superior performance at 55 °C compared to other brands . Therefore MQ RScript reverse transcriptase does not encounter significant higher Ct values compared to other brands due to their low amounts of input cDNA .
Product Name
MQ Reverse Transcriptase
Catalog Number
EN-M-005-250
Lysozyme
Lysozyme is a glycoside hydrolase enzyme that is biologically active and consists of ~130 amino acid residues with four S-S bonds and a molecular weight of 14,300 Da. It can be used to hydrolyze the cell walls of Gram-positive and Gram-negative bacteria. Lysozyme has a good lytic effect on Gram-positive bacteria, aerophilic spore-forming bacteria, Bacillus subtilis, and Micrococcus radioresistant, and also has a certain lytic effect on Gram-negative bacteria such as Escherichia coli, Mutans common, and Vibrio parahaemolyticus. Lysozyme has a special role in decomposing peptidoglycans in bacterial cell walls. This product is soluble in water, insoluble in diethyl ether and acetone, can exist stably in acidic medium, and is easy to be inactivated in alkaline medium. It is easy to be inhibited and damaged by heavy metal ions (Fe³⁺, Cu²⁺, Hg⁺, Pb²⁺, etc.) and oxidants.
Product Name
Lysozyme
Catalog Number
EN-M-006-5
Trypsin
The commonly used digests are trypsin, EDTA, etc. Their main function is to hydrolyze proteins (such as extracellular matrix) between cells, disperse tissues or adherent cells into single cells, and make cell suspension for further experiments.
Notes
1. Due to the different properties of tissues or cells, the end-user should determine the optimal digestion time according to the specific procedure. The time of digesting cells should not be too long, otherwise, it will affect the cell adhesion and growth.
2. This product does not contain bacteriostatic agents; in the use of the process, special attention should be paid to aseptic operation to avoid microbial contamination.
3. It is not suitable for long-term storage at 4°C; avoid repeated freezing and thawing, and it is recommended to separate and freeze when used in small amounts.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
Product Name
Trypsin
Catalog Number
EN-M-007-5
Taq DNA Polymerase
Taq DNA Polymerase is a thermostable DNA polymerase that catalyzes the polymerization of nucleotides into duplex DNA in the 5 ’ → 3 ’ direction . MOLEQULE - ON’s Taq DNA polymerase is isolated from E . coli strain containing the Taq DNA polymerase gene from Thermus aquaticus . The buffer system is optimized for high specificity and guarantees minimal by - product formation . Usually 1 - 1 . 5 u of Taq DNA Polymerase is used in 50 μl of reaction mix . Higher Taq DNA Polymerase concentrations may cause synthesis of nonspecific products . However, if inhibitors are present in the reaction mix (e . g . , if the template DNA used is not highly purified), higher amounts of Taq DNA Polymerase ( 2 - 3 U) may be necessary to obtain a better yield of amplification products . This product can be used in Routine PCR, SYBR - Green - based qPCR , Dual - labeled probe based qPCR , primer extension, TA cloning and gene sequencing.
MQ HotStart NanoTaq DNA Polymerase
MOLEQULE - ON HotStart NanoTaq DNA Polymerase, is an enhanced hot start enzyme DNA Polymerase engineered with nano technology, which differentiates from the traditional methods of hot start enzymes . It provides the convenience and reliability towards your research destination . MQ NanoTaq covers reactions at room temperature and cycling conditions (using same protocol) as the conventional Taq as well as reducing nonspecific primer annealing, improving product yield and ideal for PCR products application of up to 5 kb.
Figure 1 . Comparison of non - specific amplifying effects . All amplifications were performed in accordance with manufacturer’s instructions to amplify 320 to 941 bp amplicons from human genomic DNA . MQ NanoTaq provided both higher specificity and yield products with least band shifting compared to other commercial antibody - mediated hot start polymerase.
Figure 2 . Sensitivity and reliable amplification from low amounts of input DNA . Amplification of a 803 bp fragment from 3 ; 0 . 3 ; 0 . 2 ; 0 . 1 ; 0 . 03 ; 0 (no template control) ng of human genomic DNA were amplified in 20 μL PCR reactions using MQ NanoTaq DNA Polymerase.
Figure 3 . Efficient amplification of DNA sequences with a range of GC content . A series of DNA fragments of increasing GC content were amplified from human gDNA . MQ HotStart NanoTaq DNA Polymerase was used for targets with > 50 % GC.
PR – M – 003 – 500
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